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Purified Mouse Anti-Human BUBR1
Purified Mouse Anti-Human BUBR1

Western blot analysis of BUBR1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-human BUBR1 antibody.

Purified Mouse Anti-Human BUBR1

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of BUBR1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-human BUBR1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human BUBR1 aa. 276-388
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
125 kDa
250 µg/ml
AB_2066085
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612502 Rev. 1
Antibody Details
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9/BUBR1

Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately atttached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. BUBR1 is a bub-related kinase homologous to Bub1 and MAD3. BUBR1 contains a nuclear localization signal and two highly conserved domains CD1 and CD2. CD1 is in the N-terminal region and is invoved in kinetochore localization, while CD2 is in the C-terminal region and is involved in kinase activity. BUBR1 is hyperphosphorylated during spindle checkpoint activation, and interacts with p55CDC and APC during spindle checkpoint function. In colorectal cancers, the BUBR1 gene has structural abnormalities that suggest BUBR1 is a tumor suppressor gene. Thus, BUBR1 may be a critical spindle checkpoint kinase and disruption of its function may be related to tumor formation.

612502 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612502 Rev.1
Citations & References
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Development References (3)

  1. Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol. 1999; 146(5):941-954. (Biology). View Reference
  2. Mizunuma M, Hirata D, Miyahara K, Tsuchiya E, Miyakawa T. Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast. Nature. 1998; 392(6673):303-306. (Biology). View Reference
  3. Wu H, Lan Z, Li W, et al. p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase. Oncogene. 2000; 19(40):4557-4562. (Biology). View Reference
612502 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.