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Purified Mouse Anti-Fatty Acid Synthase
Purified Mouse Anti-Fatty Acid Synthase

Western blot analysis of Fatty Acid Synthase on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-FAS antibody.

Purified Mouse Anti-Fatty Acid Synthase

Immunofluorescent staining of MDCK cells with anti-FAS antibody.

Western blot analysis of Fatty Acid Synthase on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-FAS antibody.

Immunofluorescent staining of MDCK cells with anti-FAS antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Rabbit (Tested in Development)
Mouse IgG1
Human Fatty Acid Synthase aa. 9-202
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
265 kDa
250 µg/ml
AB_398276
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610962 Rev. 1
Antibody Details
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23/Fatty Acid Synthase

Fatty acid biosynthesis occurs in all living organisms and provides essential components of biological membranes as well as a form of energy storage. Animal fatty acid synthase (FAS) is a multifunctional enzyme that catalyzes the synthesis of long-chain fatty acids via sequential condensation of two-carbon units from malonyl-CoA, an intermediate derived from the carboxylation of Acetyl-CoA. FAS is a homodimer of a multifunctional subunit protein that contains seven distinct activities and a site for the prosthetic group 4'-phosphopantetheine (acyl carrier protein). These domains are oriented from N-terminus to C-terminus as follows: β-keto-acyl synthase, acetyl and malonyl transacylases, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase. Although all domains are found on each subunit, they are only active following the homodimerization of subunits in an antiparallel (head-to-tail) orientation. This juxtaposition and cooperation between domains forms two centers for acyl chain assembly. Alternative substrates and chain-terminating mechanisms allow for the production of a variety of fatty acids with different lengths and structures.

610962 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610962 Rev.1
Citations & References
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Development References (3)

  1. Chirala SS, Huang WY, Jayakumar A, Sakai K, Wakil SJ. Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities. Proc Natl Acad Sci U S A. 1997; 94(11):5588-5593. (Biology). View Reference
  2. Jayakumar A, Tai MH, Huang WY, et al. Human fatty acid synthase: properties and molecular cloning. Proc Natl Acad Sci U S A. 1995; 92(19):8695-8699. (Biology). View Reference
  3. Smith S. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB J. 1994; 8(15):1248-1259. (Biology). View Reference
610962 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.