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Purified Mouse Anti-Ezrin
Product Details
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BD Transduction Laboratories™
Dog (QC Testing), Human (Tested in Development)
Mouse IgG1
Human Ezrin aa. 391-515
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
80 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610603 Rev. 1
Antibody Details
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First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.

610603 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610603 Rev.1
Citations & References
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Development References (5)

  1. Anastasiadis PZ, Moon SY, Thoreson MA, et al. Inhibition of RhoA by p120 catenin. Nat Cell Biol. 2000; 2(9):637-644. (Clone-specific: Immunofluorescence). View Reference
  2. Berryman M, Gary R, Bretscher A. Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis. J Cell Biol. 1995; 131(5):1231-1242. (Biology). View Reference
  3. Defacque H, Egeberg M, Habermann A, et al. Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO J. 2000; 19(2):199-212. (Clone-specific: Western blot). View Reference
  4. Mohler PJ, Kreda SM, Boucher RC, Sudol M, Stutts MJ, Milgram SL. Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50. J Cell Biol. 1999; 147(4):879-890. (Clone-specific: Immunofluorescence). View Reference
  5. Perez OD, Kinoshita S, Hitoshi Y, et al. Activation of the PKB/AKT pathway by ICAM-2. Immunity. 2002; 16(1):51-65. (Clone-specific: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610603 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.