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Purified Mouse Anti-EZH2
Purified Mouse Anti-EZH2

Western blot analysis of EZH2 expression on Jurkat cell lysate. Jurkat cell lysate (Cat. No. 611451) was stained with Purified Mouse Anti-EZH2 (Cat. No. 612667/612666) at 1:1000 (Lane 1), 1:2000 (Lane 2), and 1:4000 (Lane 3) dilutions. EZH2 expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).

Purified Mouse Anti-EZH2

Immunofluorescent staining of EZH2 expression on Hela cells. Hela cells were fixed with formaldehyde, then stained with Purified Mouse Anti-EZH2. EZH2 expression was visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).

Western blot analysis of EZH2 expression on Jurkat cell lysate. Jurkat cell lysate (Cat. No. 611451) was stained with Purified Mouse Anti-EZH2 (Cat. No. 612667/612666) at 1:1000 (Lane 1), 1:2000 (Lane 2), and 1:4000 (Lane 3) dilutions. EZH2 expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).

Immunofluorescent staining of EZH2 expression on Hela cells. Hela cells were fixed with formaldehyde, then stained with Purified Mouse Anti-EZH2. EZH2 expression was visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).

Product Details
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BD Transduction Laboratories™
Enhancer of zeste 2; ENX-1; Histone-lysine N-methyltransferase EZH2; KMT6
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human EZH2 recombinant protein aa. 156-256
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
91 kDa
250 µg/ml
AB_399911
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
612666 Rev. 3
Antibody Details
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11/EZH2

The 11/EZH2 monoclonal antibody specifically binds to the methyltransferase, EZH2 (Enhancer of Zeste Homolog 2). EZH2 is a human homologue of Drosophila's Enhancer of zeste gene, an important regulator of homeobox gene expression. The EZH2 protein has a predicted molecular weight of ~85 kDa. EZH2 is a member of the Polycomb group (PcG) of proteins that are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG family proteins form multimeric complexes that regulate the expression of genes involved in cell cycle, DNA repair and differentiation. Specifically, EZH2 is a core enzymatic component of PRC2 (polycomb repressive complex 2). EZH2 is expressed in some lymph node follicular T cells and B cells. Thymocytes differentially express EZH2 at various stages during T-cell maturation. EZH2 interacts with multiple signaling proteins, including Vav, that are involved in lymphocyte development and activation. It is highly expressed in a variety of tumors including lymphomas as well as breast and prostate cancers. EZH2 is important in the self renewal and proliferation of numerous stem cell types including fetal hematopoietic stem cells, muscle satellite cells, hepatic stem/progenitor cells, neural stem cells, basal cell progenitors in the developing epidermis, embryonic stem cells, and some cancer stem cells.

612666 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612666 Rev.3
Citations & References
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Development References (3)

  1. Fujii S, Ito K, Ito Y, Ochiai A.. Enhancer of zeste homologue 2 (EZH2) down-regulates RUNX3 by increasing histone H3 methylation. J Biol Chem. 2008; 283(25):17324-17332. (Clone-specific). View Reference
  2. Raaphorst FM, Otte AP, van Kemenade FJ. Distinct BMI-1 and EZH2 expression patterns in thymocytes and mature T cells suggest a role for Polycomb genes in human T cell differentiation. J Immunol. 2001; 166(10):5925-5934. (Biology). View Reference
  3. van Kemenade FJ, Raaphorst FM, Blokzijl T. Coexpression of BMI-1 and EZH2 polycomb-group proteins is associated with cycling cells and degree of malignancy in B-cell non-Hodgkin lymphoma. 2001; 97(12):3896-3901. (Biology). View Reference
612666 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.