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Purified Mouse Anti-EB1
Purified Mouse Anti-EB1
Western blot analysis of EB1 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- EB1 antibody.
Purified Mouse Anti-EB1

Immunofluorescence staining of human intestinal smooth muscle cells (HISM).

Western blot analysis of EB1 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- EB1 antibody.

Immunofluorescence staining of human intestinal smooth muscle cells (HISM).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
Mouse IgG1
Human EB1 aa. 107-268
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-paraffin (Tested During Development), Immunoprecipitation (Not Recommended)
30 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610535 Rev. 1
Antibody Details
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Familial adenomatous polyposis is an autosomal inherited disease characterized by mutations in the APC (adenomatous polyposis coli) gene. Individuals with this disease are predisposed to colorectal polyps and cancer. It has been proposed that disruption of the APC gene plays a role in the disease. APC is a large coiled-coil protein that interacts, via its carboxy terminus, with catenins and with the protein, EB1. This 30 kDa protein was discovered as a result of its strong binding to the C-terminal domain of APC. Like the APC-binding proteins β-Catenin and pp120Cas, EB1 may play an essential role in modulating APC activity and tumorigenesis.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610535 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (5)

  1. Askham JM, Vaughan KT, Goodson HV, Morrison EE. Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Mol Biol Cell. 2002; 13(10):3627-3645. (Biology: Immunofluorescence, Western blot). View Reference
  2. Etienne-Manneville S, Hall A. Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity. Nature. 2003; 421(6924):753-756. (Biology: Immunofluorescence). View Reference
  3. Komarova YA, Akhmanova AS, Kojima S, Galjart N, Borisy GG. Cytoplasmic linker proteins promote microtubule rescue in vivo. J Cell Biol. 2002; 159(4):589-599. (Biology: Immunofluorescence, Western blot). View Reference
  4. Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Western blot). View Reference
  5. Su SL, Huang IP, Fair WR, Powell CT, Heston WD. Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression. Cancer Res. 1995; 55(7):2972-2977. (Biology). View Reference
View All (5) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.