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Purified Mouse Anti-DLP1
Purified Mouse Anti-DLP1

Western blot analysis of DLP1 on a rat cerebrum lysate. 1 µg/mL (lane 1), 0.5 µg/mL (lane 2) and 0.25 µg/mL (lane 3) of the mouse anti-DLP1 antibody.  

Purified Mouse Anti-DLP1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of DLP1 on a rat cerebrum lysate. 1 µg/mL (lane 1), 0.5 µg/mL (lane 2) and 0.25 µg/mL (lane 3) of the mouse anti-DLP1 antibody.  

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Dynamin Like Protein-1; DVLP
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Rat DLP1 aa. 601-722
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
79-84 kDa
250 µg/ml
AB_398424
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611112 Rev. 1
Antibody Details
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8/DLP1

Dynamins, phosphoproteins with intrinsic GTPase activities, play an important role during early endocytosis.  The GTPase activity is stimulated in vitro by microtubules, SH3 domain containing-proteins, and Protein Kinase (PKC)- mediated phosphorylation.  Although highly related, dynamins are differentially expressed in an organism.  For instance, dynamin I is found only in the nervous system, dynamin II is ubiquitously expressed, and dynamin III is located in lung, testis and brain.  Due to four alternative splicing products for each of the dynamins, the dynamin family contains at least 12 different members.  The Dynamin Like Protein-1 (DLP1) is a new member of the dynamin family of  proteins.  DLP1 (also known as DVLP) is widely expressed as two alternatively spliced products, each with different tissue expression patterns.  The larger product is about 84 kDa and reportedly found only in the brain, whereas the 79 kDa product is ubiquitously expressed.  Unlike dynamins I-III which co-localize with endocytotic vesicles, DLP1 is found aligned with microtubules and with the endoplasmic reticulum, suggesting a secretory function for this novel protein.

611112 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611112 Rev.1
Citations & References
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Development References (6)

  1. Bossya B, Bossy-Wetzela E, Petrillia A, et al. S-Nitrosylation of DRP1 Does Not Affect Enzymatic Activity and is Not Specific to Alzheimer’s Disease. 2010; 20:S513-S526. (Biology: Western blot). View Reference
  2. Duvezin-Caubet S, Reichert AS, Jagasia R, et al. Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem. 2006; 281(49):37972-37979. (Biology: Western blot). View Reference
  3. Estaquier J, Arnoult D. Inhibiting Drp1-mediated mitochondrial fission selectively prevents the release of cytochrome c during apoptosis. Cell Death Differ. 2007; 14(6):1086-1094. (Biology: Western blot). View Reference
  4. Jofuku A, Ishihara N, Mihara K. Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein. Biochem Biophys Res Commun. 2005; 333(2):650-659. (Biology: Western blot). View Reference
  5. Karbowski M, Neutzner A, Youle RJ. The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division. J Cell Biol. 2007; 178(1):71-84. (Biology: Immunofluorescence, Western blot). View Reference
  6. Yoon Y, Pitts KR, Dahan S, McNiven MA. A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. J Cell Biol. 1998; 140(4):779-793. (Biology). View Reference
View All (6) View Less
611112 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.