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Purified Mouse Anti-CtBP1
Purified Mouse Anti-CtBP1

Western blot analysis of CtBP1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-CtBP1 antibody.

Purified Mouse Anti-CtBP1

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of CtBP1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-CtBP1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
C-terminal Binding Protein-1
Mouse (QC Testing), Human, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Mouse CtBP1 aa. 345-441
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
48 kDa
250 µg/ml
AB_399429
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612042 Rev. 3
Antibody Details
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3/CtBP1

Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription regulator that has an intrinsic repressor domain, or a transcription regulator that contains a binding site in the regulatory domain where a corepressor can bind. C-terminal binding proteins (CtBP1 and CtBP2) are a class of corepressors that interact with a subset of transcription factors through a PLDLSL sequence motif. CtBP1 and CtBP2 are 80% homologous, and both are expressed at high levels in embryonic tissues. However, CtBP1 is expressed higher in adult tissues, and is more widely expressed in embryonic and adult tissues. Both CtBP1 and CtBP2 bind the δEF1 zinc finger-homeodomain transcription factor, and enhance transcriptional repression via interaction with the PLDLSL-sequence in δEF1. CtBP1 and CtBP2 bind other zinc finger transcription factors, including Kheper and BKLF. In addition, CtBP2 interacts with other transcription factors, such as hFOG-2, Evi-1, AREB6, and ZEB. Thus, CtBP1 and CtBP2 may be important corepressors for a variety of transcriptional factors.

612042 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612042 Rev.3
Citations & References
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Development References (4)

  1. Furusawa T, Moribe H, Kondoh H, Higashi Y. Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1. Mol Cell Biol. 1999; 19(12):8581-8590. (Biology). View Reference
  2. Hildebrand JD, Soriano P. Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development. Mol Cell Biol. 2002; 22(15):5296-5307. (Biology: Immunohistochemistry, Western blot). View Reference
  3. Muraoka O, Ichikawa H, Shi H. Kheper, a novel ZFH/deltaEF1 family member, regulates the development of the neuroectoderm of zebrafish (Danio rerio). Dev Biol. 2000; 228(1):29-40. (Biology). View Reference
  4. Schaeper U, Boyd JM, Verma S, Uhlmann E, Subramanian T, Chinnadurai G. Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation. Proc Natl Acad Sci U S A. 1995; 92(23):10467-10471. (Biology). View Reference
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612042 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.