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Western blot analysis of CIP4 on JAR cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-CIP4 antibody.
Immunofluorescent staining of NRK cells.
BD Transduction Laboratories™ Purified Mouse Anti-CIP4
BD Transduction Laboratories™ Purified Mouse Anti-CIP4
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. Similarly, CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42-interacting protein 4 (CIP4) was identified in a yeast-two hybrid screen for proteins that bind CDC42. Another variant of CIP4, CIP4/2, was identified that contains an extra 56 amino acids and has 71% identity with CIP4 (or CIP4/1). CIP4 contains a C-terminal SH3 domain and an N-terminal domain that is homologous to non-catalyitic motifs in the tyrosine kinase Fer. The mRNA expression of CIP4 is highest in skeletal muscle, heart, and placenta. Overexpression of CIP4 in Swiss 3T3 cells reduces the amount of stress fibers and leads to clustering of CIP4 to foci at the dorsal side of the cells. In addition, CIP4 binds the Rho-GTPase activating protein RICH and the cytoskeletal protein WASP. Coexpression of CIP4 and WASP in Cos-7 cells leads to WASP association with microtubules. Thus, CIP4 is involved in various protein-protein interactions associated with cytoskeletal dynamics.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Aspenstrom P. A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton. Curr Biol. 1997; 7(7):479-487. (Biology). View Reference
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Richnau N, Aspenstrom P. Rich, a rho GTPase-activating protein domain-containing protein involved in signaling by Cdc42 and Rac1. J Biol Chem. 2001; 276(37):35060-35070. (Biology). View Reference
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Tian L, Nelson DL, Stewart DM. Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules. J Biol Chem. 2000; 275(11):7854-7861. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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