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Purified Mouse Anti-CD100
Purified Mouse Anti-CD100

Western blot analysis of CD100 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). 1 µg/mL (lane 1), 0.5 µg/mL (lane 2), 0.25 µg/mL (lane 3) of the mouse anti-CD100 antibody.

Purified Mouse Anti-CD100

Immunofluorescence staining of rabbit spleen.

Western blot analysis of CD100 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). 1 µg/mL (lane 1), 0.5 µg/mL (lane 2), 0.25 µg/mL (lane 3) of the mouse anti-CD100 antibody.

Immunofluorescence staining of rabbit spleen.

Product Details
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BD Transduction Laboratories™
Semaphorin 4D; Sema 4D
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human CD100 aa. 721-861
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
150 kDa
250 µg/ml
AB_397998
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610671 Rev. 2
Antibody Details
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30/CD100

CD100 was originally described as a 150 kDa surface T-cell antigen induced after T-cell activation.  Human CD100 exhibits widespread gene expression but is most prominent in peripheral lymphocytes, thymus, skeletal muscle, and brain.  The full CD100 protein is 862 amino acids with homolgy to the semaphorin family of proteins at its extracellular domain followed by a short Ig-like domain.  The short cytoplasmic domain contains a putative tyrosine phosphorylation site which may be the site for association with intracellular kinases.  Cells that overexpress CD100 induce a rapid aggregation of B cells and increase the CD90L-B cell aggregation.  Because semaphorins are axon-guiding molecules for the nervous system, it has been proposed that CD100 may have a guidance role in the immune system.

610671 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610671 Rev.2
Citations & References
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Development References (1)

  1. Hall KT, Boumsell L, Schultze JL, et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci U S A. 1996; 93(21):11780-11785. (Biology). View Reference
610671 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.