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Purified Mouse Anti-Actin Ab-5
Purified Mouse Anti-Actin Ab-5

Western blot analysis of Actin Ab-5 on Jurkat cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Actin Ab-5.

Purified Mouse Anti-Actin Ab-5

Immunofluorescent staining of Hs68 cells with anti-Actin Ab-5.

Western blot analysis of Actin Ab-5 on Jurkat cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Actin Ab-5.

Immunofluorescent staining of Hs68 cells with anti-Actin Ab-5.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1, κ
Chicken gizzard muscle Actin
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
42 kDa
250 µg/ml
AB_2289199
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612657 Rev. 3
Antibody Details
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C4/actin

Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments results from interactions between actin and actin-binding proteins.  Actin is a 42-kDa protein that is known as G-actin in its monomeric form.  Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin.  F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks.  The latter are highly concentrated beneath the plasma membrane and form the actin cortex.  Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins.  These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures.  Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins.

The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.  

612657 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612657 Rev.3
Citations & References
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Development References (4)

  1. Hanstein C, Lange U, Schneider-Poetsch HA, Grolig F, Wagner G. Detection of actin and localization of phytochrome in the green alga Mougeotia by monocloanl antibodies. Acta Histochem Suppl. 1991; 41:223-230. (Biology). View Reference
  2. Lesssard JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton. 1988; 10(3):349-362. (Biology). View Reference
  3. Mitchison TJ, Cramer LP. Actin-based cell motility and cell locomotion. Cell. 1996; 84(3):371-379. (Biology). View Reference
  4. Pantaloni D, Le Clainche C, Cartier M-F. Mechanism of Actin-Based Motility. Science. 2001; 292:1502-1506. (Biology).
View All (4) View Less
612657 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.