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Purified Rat Anti-Mouse IgE
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse IgE (pooled)
ELISA Capture (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Sandwich ELISA:  Purified rat anti-mouse IgE (clone R35-92) may be used at ~ 2 µg/ml as the capture antibody coupled with biotin rat anti-mouse IgE (clone R35-72) (Cat. No. 553414) as the detection antibody.  Researchers are strongly advised to titrate each reagent over a range of concentrations (e.g 1-8 µg/ml) for optimal results.  Purified mouse IgE (Cat. No. 557079, 553481, or 557080) may be used as the ELISA standard.  Researchers, however, are advised to first consider using purified rat anti-mouse IgE (clone R35-72) (Cat. No. 553413) and biotin rat anti-mouse IgE (clone R35-118) (Cat. No. 553419) as the ELISA sandwich pair for measuring mouse IgE where greater sensitivity is needed.  Alternatively, the BD OptEIA™ Mouse IgE ELISA Set (Cat. No. 555248) is offered as a convenient sandwich ELISA product that is easy-to-use and may be used for the quantitation of soluble mouse IgE.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553416 Rev. 12
Antibody Details
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The rat anti-mouse IgE antibody (clone R35-92) reacts specifically with mouse IgE of Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes.

This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553416 Rev. 12
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553416 Rev.12
Citations & References
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View product citations for antibody "553416" on CiteAb
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553416 Rev. 12

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.