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Purified Mouse Anti-Rabbit IgG
Product Details
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BD Pharmingen™
Rabbit (QC Testing), Human,Mouse,Rat,Guinea Pig (Lack of Reactivity Confirmed in Development)
Mouse BALB/c IgG1, κ
ELISA Capture (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

We recommend using the purified C101-359 antibody for capture in sandwich rabbit IgG ELISA in combination with biotinylated anti-rabbit IgG C101-167 mAb (Cat. no. 550346) for detection and purified rabbit IgG (Cat. no. 550875) as a standard.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550326 Rev. 6
Antibody Details
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The C101-359 antibody reacts with rabbit IgG, but not with rabbit IgM. It does not cross-react with human, rat, mouse, or guinea pig immunoglobulins.

550326 Rev. 6
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550326 Rev.6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.