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BD Pharmingen™ Purified Mouse Anti-Human IP-10
Clone 4D5/A7/C5 (RUO)
Human IP-10 ELISA Standard Curve
Human IP-10 ELISA Standard Curve
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG2a, λ
Recombinant Human IP-10 protein
ELISA Capture (Routinely Tested)
0.5 mg/ml
AB_395668
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures
ELISA Capture: The purified monoclonal antibody 4D5/A7/C5 is useful as a capture antibody in a sandwich ELISA for measuring human IP-10 protein levels. Purified monoclonal antibody 4D5/A7/C5 can be paired with the biotinylated monoclonal antibody 6D4/D6/G2 (Cat. No. 555048) with recombinant human IP-10 (Cat. No. 551130) as the standard. Purified monoclonal antibody 4D5/A7/C5 should be titrated to determine its optimal concentration for ELISA capture (0.5-2.0 µg/ml). To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~2500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, w ww.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (human IL-1α, IL-1αβ, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, lymphotactin, MIG, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TGF, TNF, LT-β and mouse Crg-2).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring IP-10 in serum or plasma the BD OptEIA™ Human IP-10 ELISA Set (Cat. No. 550926 ) is specially formulated and recommended.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555046 Rev. 1
Antibody Details
4D5/A7/C5
The monoclonal antibody 4D5/A7/C5 reacts with human CXC chemokine, interferon gamma inducible protein (IP-10). IP-10 is inducible in monocytes, keratinocytes, and endothelial cells by IFN-γ.
This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
555046 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555046 Rev.1
Citations & References
View product citations for antibody "555046" on CiteAb
Development References (1)
-
Luster AD, Ravetch JV. Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). J Exp Med. 1987; 166(4):1084-1097. (Clone-specific). View Reference
555046 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.