Skip to main content Skip to navigation
Purified Mouse Anti-Human CD95
Purified Mouse Anti-Human CD95

Western blot analysis of recombinant FasÄTM. Purified mouse anti-human Fas antibody (clone G254-274) was used to stain 200 ng of recombinant FasÄTM (MN 554336).

Western blot analysis of recombinant FasÄTM. Purified mouse anti-human Fas antibody (clone G254-274) was used to stain 200 ng of recombinant FasÄTM (MN 554336).

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Fas/APO-1
Human (QC Testing)
Mouse IgG1
Recombinant Human Fas
Western blot (Routinely Tested), ELISA Capture (Tested During Development), Flow cytometry, Functional assay (Not Recommended)
45 kDa
0.5 mg/ml
AB_396391
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2µg/ml). Clone G254-274 has also been tested in antibody development for ELISA where G254-274 has been used as a capture antibody paired with the biotin mouse anti-human Fas clone DX2 (MN 555672) as the detection antibody. The BD OptEIA™ Set for soluble, human Fas (MN 555224) is the suggested format for use in ELISA analysis. Clone G254-274 is not suggested for flow cytometric analysis or for use in functional apoptosis assays.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556370 Rev. 5
Antibody Details
Down Arrow Up Arrow
G254-274

Programmed cell death (apoptosis) may be induced in response to a variety of  cytotoxic stimuli, including activation of surface receptors. An area of particular interest has been the induction of apoptosis by the receptor-ligand pair: Fas (CD95) and Fas Ligand (FasL). Fas is an ~45 kD cell surface protein which  belongs to the the TNF (tumor necrosis factor receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL, a member of the TNF ligand family, is expressed on activated T and NK cells. FasL initiates signaling at the cell surface by aggregation of individual Fas recpetors via binding to the multivalent ligand. Antibodies which selectively activate Fas can be used in vitro to mimic the apoptotic response associated with FasL.  Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.  Clone G254-274 recognizes human Fas. A soluble form of recombinant human Fas (lacking the Fas transmembrane domain) was used as immunogen.

556370 Rev. 5
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556370 Rev.5
Citations & References
Down Arrow Up Arrow

Development References (2)

  1. Takahashi T, Tanaka M, Brannan CI, et al. Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell. 1994; 76(6):969-976. (Biology). View Reference
  2. Tanaka M, Suda T, Takahashi T, Nagata S. Expression of the functional soluble form of human Fas ligand in activated lymphocytes. EMBO J. 1995; 14(6):1129-1135. (Biology). View Reference
556370 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00