ELISA Detection: The biotinylated MP1-31G6 antibody (Cat. No. 554407) is useful as a detection antibody for a sandwich ELISA for measuring mouse GM-CSF protein levels. Biotinylated MP1-31G6 antibody can be paired with the purified MP1-22E9 antibody (Cat. No. 554404) as the capture antibody, with recombinant mouse GM-CSF (Cat. No. 554586) as the standard. This detecting antibody should be titrated from 0.5 -2.0 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of mouse GM-CSF protein ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouseIL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1ß, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: For measuring cytokine in plasma or serum our BD OptEIA™ mouse GM-CSF ELISA Set, Cat. No. 555167 is specially formulated and recommended.
IP/WB: The MP1-31G6 antibody has been reported to be useful for immunoprecipitation studies. Please note that this application is not routinely tested at BD Biosciences Pharmingen.