Biotin Mouse Anti-Pig IFN-γ

BD Pharmingen™ Biotin Mouse Anti-Pig IFN-γ

Name: Biotin Mouse Anti-Pig IFN-γ
Brand: BD Pharmingen™
SKU: 559958

Clone P2C11

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Pig (QC Testing)

Isotype: Mouse IgG2a, κ

Immunogen: Purified recombinant pig IFN-γ

Application: ELISA Detection (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_397394

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA Detection: The biotinylated P2C11 antibody (Cat. No. 559958) is useful as a detection antibody for a sandwich ELISA that measures pig IFN-γ protein levels. Biotinylated P2C11 antibody can be paired with the purified P2G10 antibody (Cat. No. 559961) as the capture antibody, with recombinant pig IFN-γ as the standard. Biotin P2C11 antibody should be titrated (0.25 - 1.0 µg/ml) to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant pig IFN-γ protein ranging from ~200 to 1.5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Note 1: This ELISA pair shows no cross-reactivity with mouse, rat and human IFN-γ.

Note 2: This ELISA pair is recommended primarily for measuring cytokines generated by experimental cell culture systems. These ELISA reagents are not recommended for detection in serum samples.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

559958 Rev. 2

Antibody Details

P2C11

The P2C11 antibody reacts with pig IFN-γ protein. The immunogen used to generate the P2C11 hybridoma was purified recombinant pig IFN-γ.

559958 Rev. 2

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

559958 Rev.2

Citations & References

Development References (1)

Mateu de Antonio E, Husmann RJ, Hansen R, et al. Quantitative detection of porcine interferon-gamma in response to mitogen, superantigen and recall viral antigen. Vet Immunol Immunopathol. 1998; 61(2-4):265-277. (Biology: ELISA). View Reference

559958 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.