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BD™ Cytometric Bead Array (CBA) Mouse IL-23 p19/p40 Flex Set
Product Details
BD™ Cytometric Bead Array (CBA)
10-2,500 pg/mL
Mouse (QC Testing)
RUO
AB_2869425
Description
The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is a bead-based immunoassay capable of measuring mouse Interleukin-23 (p19/p40) in cell culture supernatant samples. Investigators are encouraged to titrate cell culture supernatant samples, such as with a 1:2 dilution with CBA Assay Diluent, prior to testing. The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is not recommended for testing mouse serum or plasma samples. Mouse reactivity was determined by testing samples with the BD CBA Mouse IL-23 (p19/p40) Flex Set. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).
Preparation And Storage
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures
The BD CBA Mouse IL-23 (p19/p40) Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions for the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Mouse IL-23 (p19/p40) Flex Set will be 2,500 pg/mL.
The BD CBA Mouse IL-23 (p19/p40) Flex Set should not be used in the same assay well with any non-BD CBA Mouse Soluble Protein Flex Set Reagents, which include: Human Soluble Protein, Human Enhanced Sensitivity, Mouse Enhanced Sensitivity, Human Soluble Receptor and Cell Signalling Flex Sets. For an updated assay compatibility chart for the BD CBA Mouse Soluble Protein Flex Sets, please visit http://www.bdbiosciences.com/cbasetup.
Performance
Limit of Detection: The theoretical limit of detection is 1.04 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.
Product Notices
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
Companion Products
Mouse/Rat Soluble Protein Master Buffer Kit RUO
Size
100 Tests
Cat No.
558266
Mouse/Rat Soluble Protein Master Buffer Kit RUO
Size
500 Tests
Cat No.
558267
562575 Rev. 4
Components
| Description | Quantity/Size | Part Number |
|---|---|---|
| Mouse IL-23 p19/p40 Capture Bead D7 | N/A | 51-9008106 |
| N/A | N/A | 51-9008108 |
| N/A | N/A | 51-9008110 |
562575 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.