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Human TNF Flex Set

Human TNF Flex Set

(RUO)
Human TNF Flex Set

Figure 1. Example BD CBA Human TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analzed using the FCAP Array Software (Cat. No. 641488).

Human TNF Flex Set

Recovery:        Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Human TNF Flex Set

Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Human TNF Flex Set

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

Figure 1. Example BD CBA Human TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analzed using the FCAP Array Software (Cat. No. 641488).

Recovery:        Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

Product Details
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BD™ Cytometric Bead Array (CBA)
C4
10-2,500 pg/mL
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
RUO
AB_2869306


Description

The BD™ CBA Human TNF Flex Set is a bead-based immunoassay capable of measuring human tumor necrosis factor (TNF), formerly known as tumor necrosis factor-α, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human TNF Flex Set. The biology and function of TNF has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).

Preparation And Storage

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Human TNF Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human TNF Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1.

The BD CBA Human TNF Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents:

Flex Set                                                 Bead Position                Catalog Number

BD CBA Human TNF Flex Set                        D9                             558273

BD CBA Human Angiogenin Flex Set                C4                             558328

The BD CBA Human TNF Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset.

Performance

Limit of Detection: The theoretical limit of detection is 1.2 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.

Product Notices

  1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  2. ProClin is a trademark of Rohm and Haas Company.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
560112 Rev. 3
Components
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Description Quantity/Size Part Number
Human TNF Capture Bead C4 N/A 51-9005468
Human TNF PE Detection Reagent N/A 51-9004040
Human TNF Standard 1 Vial(s) (2 ea) 51-9003509
560112 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.