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R718 Mouse Anti-Mouse Vβ 17[a] T-Cell Receptor
Product Details
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BD OptiBuild™
T cell receptor beta variable 17[a]; TCR Vb17[a]
Mouse (Tested in Development)
Mouse BALB/c IgG2a, κ
Mouse T cells
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  6. Please refer to to access safety data sheets (SDS).
  7. Please refer to for technical protocols.
  8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email:
751964 Rev. 1
Antibody Details
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The KJ23 monoclonal antibody specifically recognizes Vβ 17[a] T-cell Receptor (TCR) of mice having the a haplotype (eg,C57L, SJL, SWR) of the Tcrb gene complex. Strains having the b (eg, A, AKR, BALB/c, CBA,C3H/He, C57BL, C58, DBA/1, DBA/2) Tcrb haplotype do not express functional Vβ 17 TCR, and the Tcrb-V17 gene locus is deleted in mice having the c (eg, RIII) haplotype. Vβ 17[a] TCR-bearing Tlymphocytes are clonally eliminated in mice expressing I-E (eg, C57BR). KJ23 antibody also recognizes two phenotypic variants of the Vβ 17[a] TCR: Vβ 17[a2] expressed in a variety of wild-derivedmouse strains and Vβ 17[a(cz)] expressed in Mtv-free CZ mice. The effects of Mtv-encoded superantigens upon Vβ 17[a] TCR-bearing T cells has been reviewed. Plate-bound KJ23 antibody activates Vβ 17[a] TCR-bearing T cells, and injection of the antibody can deplete Vβ 17[a]-bearing Tcells.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.

751964 Rev. 1
Format Details
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The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Red 627-640 nm
695 nm
718 nm
751964 Rev.1
Citations & References
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Development References (8)

  1. Cazenave PA, Marche PN, Jouvin-Marche E, et al. V beta 17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the V beta 17 region greatly alter T cell receptor specificity. Cell. 1990; 63(4):717-728. (Biology). View Reference
  2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
  3. Kappler JW, Roehm N, Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987; 49(2):273-280. (Biology). View Reference
  4. Kappler JW, Wade T, White J, . A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987; 49(2):263-271. (Immunogen). View Reference
  5. Katz JD, Lebow LT, Bonavida B. The in vivo depletion of V beta 17a+ T cells results in the inhibition of reticulum cell sarcoma growth in SJL/J mice. Evidence for the use of anticlonotypic antibody therapy in the control of malignancy. J Immunol. 1989; 143(4):1387-1395. (Biology). View Reference
  6. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology). View Reference
  7. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
  8. Wade T, Bill J, Marrack PC, Palmer E, Kappler JW. Molecular basis for the nonexpression of V beta 17 in some strains of mice. J Immunol. 1988; 141(6):2165-2167. (Biology). View Reference
View All (8) View Less
751964 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.