R718 Mouse Anti-Human TCR Cβ1

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BD OptiBuild™ R718 Mouse Anti-Human TCR Cβ1

Name: R718 Mouse Anti-Human TCR Cβ1
Brand: BD OptiBuild™
SKU: 752278

Clone JOVI.1

(RUO)

Multiparameter flow cytometric analysis using BD OptiBuild™ R718 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 752278) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

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Product Details

Brand: BD OptiBuild™

Alternative Name: T-cell receptor beta locus; TCRB; TRB; TRB@; TRCB1

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG2a, κ

Immunogen: Lymphoid cells from Mouse transgenic for Human HA1.7 cell TCRbeta

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Entrez Gene ID: 6957

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

Researchers should determine the optimal concentration of this reagent for their individual applications.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Alexa Fluor® is a registered trademark of Life Technologies Corporation.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate CE_IVD

Size 100 mL Cat No. 349202

R718 Mouse IgG2a, κ Isotype Control RUO

Size 50 µg Cat No. 566949

View Details

752278 Rev. 2

Antibody Details

JOVI.1

The JOVI.1 monoclonal antibody recognizes an epitope common to a large proportion of human CD4+ or CD8+ T lymphocytes that express the T cell receptor beta chain (TCRβ). The antibody was generated from a mouse immunized with transgenic mouse lymphoid cells that expressed the rearranged human Vβ3-Cβ1 TCR chain derived from the cloned human HA1.7 T helper cell. This antibody reacts with TCR-Cβ1+ T cells and one of several different TCRβ V regions, but not with TCR-Cβ2+ T cells. JOV1.1 antibody reportedly recognized several Cβ1 TCR expressing cell lines or clones including Jurkat, CH7C17, and HA1.7 cells. The JOVI.1 antibody can be used to stimulate proliferative responses by JOVI.1-positive T cells. It can also reportedly be used for immunoprecipitation and to stain JOVI.1+ T cells in frozen tissue sections.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively by for BD Biosciences as a better alternative to Alexa FluorTM 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa FluorTM 700

752278 Rev. 2

Format Details

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: R718

Excitation Source: Red 627-640 nm

Excitation Max: 695 nm

Emission Max: 718 nm

752278 Rev.2

Citations & References

Development References (3)

Gil D, Schamel WWA, Montoya M, Sanchez-Madrid F, and Alarcon B. Recruitment of Nck by CD3-epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell. 2002; 109(7):901-912. (Clone-specific: Activation, Functional assay, Immunoprecipitation). View Reference
San José E, Alarcón B. Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway.. J Biol Chem. 1999; 274(47):33740-6. (Clone-specific: Immunoprecipitation). View Reference
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ. Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992; 11(6):701-713. (Immunogen: Activation, Bioassay, Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Stimulation). View Reference

752278 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.