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Purified Mouse Anti-Human CD61
Purified Mouse Anti-Human CD61
Flow cytometric analysis of CD61 expression on human peripheral blood platelets. Platelets were stained with either Purified Mouse Anti-Human CD61 (Cat. No. 555752; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets.
Flow cytometric analysis of CD61 expression on human peripheral blood platelets. Platelets were stained with either Purified Mouse Anti-Human CD61 (Cat. No. 555752; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets.
Product Details
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BD Pharmingen™
Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3
Human (QC Testing), Rhesus,Cynomolgus,Baboon,Dog,Cow (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Western blot (Tested During Development)
0.5 mg/ml
III 866; IV P83; V T-124
AB_396093
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555752 Rev. 6
Antibody Details
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VI-PL2

The VI-PL2 monoclonal antibody specifically binds to CD61. CD61 is a 105 kDa transmembrane glycoprotein that is also known as integrin β3 and platelet glycoprotein IIIa (GPIIIa or GP3A). It is expressed on platelets, megakaryocytes, osteoclasts and endothelia. Integrin β3 associates with gpIIa (CD41) to form the CD41/CD61 complex which mediates platelet adhesion and aggregation. CD61 also associates with CD51 to form the CD51/CD61 complex (vitronectin receptor). CD61 appears to bind to fibrinogen, fibronectin, vWF, vitronectin, and thrombospondin to mediate cell adhesion.

555752 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555752 Rev.6
Citations & References
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View product citations for antibody "555752" on CiteAb

Development References (3)

  1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  2. Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
555752 Rev. 6

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.