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mIL-23R-293_overlay.png

Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R-transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PerCP-Cy5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; dashed line histogram) or PerCP-Cy5.5 Rat anti-Mouse IL-23 Receptor (Cat. No. 564828; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

564828Image1.png

Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R-transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PerCP-Cy5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; dashed line histogram) or PerCP-Cy5.5 Rat anti-Mouse IL-23 Receptor (Cat. No. 564828; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

mIL-23R-293_overlay.png 564828Image1.png Amber-Cap-Blue-Label.jpg

Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R-transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PerCP-Cy5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; dashed line histogram) or PerCP-Cy5.5 Rat anti-Mouse IL-23 Receptor (Cat. No. 564828; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R-transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PerCP-Cy5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; dashed line histogram) or PerCP-Cy5.5 Rat anti-Mouse IL-23 Receptor (Cat. No. 564828; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
Il23r; IL-23R; Interleukin 23 receptor
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse IL-23 Receptor Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738972
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  5. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
  6. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Cy is a trademark of GE Healthcare.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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564828 Rev. 2
Antibody Details
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O78-1208

The O78-1208 monoclonal antibody specifically binds to the mouse Interleukin-23 Receptor (IL-23R) subunit that is encoded by the il23r gene. The IL-23R subunit is a type I transmembrane glycoprotein and member of the hemopoietin receptor superfamily. The mouse IL-23 Receptor complex is comprised of IL-23R and IL-12 receptor beta 1 (IL-12Rβ1) subunits. The IL-23R complex can bind IL-23, a cytokine that plays roles in innate and adaptive immunity as well as in autoimmune diseases, eg, by the generation and maintenance of Th17 cells. Mouse IL-23R is expressed by activated/memory CD4+ T cells, Th1, Th2 and Th17 cells, γδ T cells, dendritic cells and macrophages as determined by IL-23R mRNA expression and IL-23R-GFP reporter mouse studies. The IL-23-bound IL-23R  complex transduces an intracellular signal pathway mediated by a Jak-STAT signaling cascade.

564828 Rev. 2
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
482 nm
676 nm
564828 Rev.2
Citations & References
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View product citations for antibody "564828" on CiteAb

Development References (6)

  1. Awasthi A, Riol-Blanco L, Jager A, et al. Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells. J Immunol. 2009; 182(10):5904-5908. (Biology). View Reference
  2. Jones LL, Alli R, Li B, Geiger TL. Differential T Cell Cytokine Receptivity and Not Signal Quality Distinguishes IL-6 and IL-10 Signaling during Th17 Differentiation.. J Immunol. 2016; 196(7):2973-85. (Clone-specific). View Reference
  3. Lankford CS, Frucht DM. A unique role for IL-23 in promoting cellular immunity. J Leukoc Biol. 2003; 73(1):49-56. (Biology). View Reference
  4. Monk JM, Hou TY, Turk HF, McMurray DN, Chapkin RS. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells.. J Nutr. 2013; 143(9):1501-8. (Clone-specific). View Reference
  5. Parham C, Chirica M, Timans J, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002; 168(11):5699-5708. (Biology: Calcium Flux). View Reference
  6. Petermann F, Rothhammer V, Claussen MC, et al. gammadelta T cells enhance autoimmunity by restraining regulatory T cell responses via an interleukin-23-dependent mechanism. Immunity. 2010; 33(3):351-363. (Biology). View Reference
View All (6) View Less
564828 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.