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    PerCP-Cy™5.5 Mouse Anti-Human CD45RA
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    Enhance your research with PerCP-Cy5.5 alternative with reduced spillover and monocyte background! Try BD Horizon RealBlue™ 705 SKU# [757395] today! More Info on RB705 #
    PerCP-Cy™5.5 Mouse Anti-Human CD45RA
    Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PerCP-Cy™5.5 Mouse Anti-Human CD45RA (Cat. No. 563429) and APC Mouse Anti-Human CD45RO (Cat. No. 559865/560899) antibodies. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two color flow cytometric dot plots show the correlated expression of CD45RA versus CD45RO for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
    PerCP-Cy™5.5 Mouse Anti-Human CD45RA
    Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PerCP-Cy™5.5 Mouse Anti-Human CD45RA (Cat. No. 563429) and APC Mouse Anti-Human CD45RO (Cat. No. 559865/560899) antibodies. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two color flow cytometric dot plots show the correlated expression of CD45RA versus CD45RO for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
    Product Details
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    BD Pharmingen™
    CD45R; PTPRC; LCA; Leukocyte common antigen
    Human (QC Testing)
    Mouse IgG2b, κ
    Flow cytometry (Routinely Tested)
    5 µl
    IV N906
    AB_2738199
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    5. Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
    6. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
    7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    8. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
    9. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
    10. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    11. This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
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    563429 Rev. 1
    Antibody Details
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    HI100

    The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naïve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.

    563429 Rev. 1
    Format Details
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    PerCP-Cy5.5
    PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    PerCP-Cy5.5
    Blue 488 nm
    482 nm
    676 nm
    563429 Rev.1
    Citations & References
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    View product citations for antibody "563429" on CiteAb

    Development References (3)

    1. Koristka S, Cartellieri M, Theil A, et al. Retargeting of human regulatory T cells by single-chain bispecific antibodies.. J Immunol. 2012; 188(3):1551-8. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
    2. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
    3. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
    563429 Rev. 1

    Please refer to Support Documents for Quality Certificates

     

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

     

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.