Flow cytometric analysis of CD36 expression on human peripheral blood platelet. Platelets were isolated from fresh whole blood and fixed with 2% formaldehyde. After washing, the fixed platelets were stained with either PerCP-Cy™5.5 Mouse Anti-Human CD36 antibody (Cat. No. 561536; solid line histogram) or with a PerCP-Cy™5.5 Mouse IgM, κ Isotype Control (Cat. No. 560857; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD36 expression on human peripheral blood platelet. Platelets were isolated from fresh whole blood and fixed with 2% formaldehyde. After washing, the fixed platelets were stained with either PerCP-Cy™5.5 Mouse Anti-Human CD36 antibody (Cat. No. 561536; solid line histogram) or with a PerCP-Cy™5.5 Mouse IgM, κ Isotype Control (Cat. No. 560857; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
GPIIIb; Platelet GPIV; OKM5-antigen; PASIV
Human (QC Testing)
Mouse IgM, κ
Human Platelet Extracts
Flow cytometry (Routinely Tested)
5 µl
IV P106
AB_10893348
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
- PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
- Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PerCP-Cy™5.5 Mouse IgM, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560857
561536 Rev. 2
Antibody Details
CB38
The CB38 monoclonal antibody specifically binds to CD36. CD36 is a 88 kDa glycoprotein IV (GPIV), the receptor for extracellular matrix proteins such as collagen and thrombospondin. CD36 is known to mediate the adhesion of Plasmodium falciparum. CD36 antigen is expressed on monocytes, platelets, endothelial cells, and some human tumor cell lines but not on lymphocytes and granulocytes. It is a very early marker of erythroid differentiation. CD36 antibody induces degranulation, release of ATP and serotonin, increase in [Ca2+]ι, and tyrosine phosphorylation of a substrate protein of 130 kDa.
561536 Rev. 2
Format Details
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
561536 Rev.2
Citations & References
View product citations for antibody "561536" on CiteAb
Development References (3)
-
Alessio M, Greco NJ, Primo L, et al. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993; 82(12):3637-3647. (Biology). View Reference
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Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F. Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991; 58:182-186. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
561536 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.