PE Rat Anti-Human IL-6

BD Pharmingen™ PE Rat Anti-Human IL-6

Name: PE Rat Anti-Human IL-6
Brand: BD Pharmingen™
SKU: 551473

Clone MQ2-6A3

(RUO)

Profile of LPS-stimulated (24 hrs) peripheral blood monocytes of rhesus macaque monkey (Macaca mulatta) analyzed by flow cytometry

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Product Details

Brand: BD Pharmingen™

Reactivity: Rhesus, Cynomolgus (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Recombinant human IL-6

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_2127751

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

551473 Rev. 3

Antibody Details

MQ2-6A3

IL-6, a 26 kDa protein, is a multifunctional cytokine secreted by both lymphoid and non-lymphoid cells. It regulates B and T cell function and hematopoiesis. The MQ2-6A3 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate the MQ2-6A3 hybridoma was recombinant human IL-6. This is a neutralizing antibody. Clone MQ2-6A3 cross-reacts with an intracellular component of peripheral blood LPS-stimulated permeabilized monocytes of rhesus and cynomolgus macaques. The reactivity pattern observed on non-human primates is similar, although weaker, than that seen on normal human monocytes.

551473 Rev. 3

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

551473 Rev.3

Citations & References

View product citations for antibody "551473" on CiteAb

Development References (2)

Villinger F, Brar SS, Mayne A, Chikkala N, Ansari AA. Comparative sequence analysis of cytokine genes from human and nonhuman primates. J Immunol. 1995; 155(8):3946-3954. (Biology). View Reference
Villinger F, Hunt D, Mayne A, Vuchetich M, Findley H, Ansari AA. Qualitative and quantitative studies of cytokines synthesized and secreted by non-human primate peripheral blood mononuclear cells. Cytokine. 1993; 5(5):469-479. (Biology). View Reference

551473 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.