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Flow cytometric analysis of SNAI2/Slug expression in human rhadomyosarcoma cells. RH-30 cells (DSMZ, ACC 489) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, dashed line) or PE Mouse anti-SNAI2/Slug (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyser.
BD Pharmingen™ PE Mouse anti-SNAI2/Slug
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The S43-1259 monoclonal antibody specifically recognizes SNAI2, also named Slug, which is a zinc finger transcriptional repressor first recognized for its roles in embryogenesis. SNAI2/Slug and other members of the snail family of transcription factors inhibit the expression of E-cadherin, which plays important roles in cell adhesion and in the maintenance of tissue structure. SNAI2/Slug activation and E-cadherin inhibition induces epithelial-mesenchymal transition (EMT) at several stages of embryonic development and also contributes to the invasiveness of malignancies. Aberrant expression of SNAI2/Slug has also been linked to cancer stem cell formation, cell cycle regulation and apoptosis. SNAI2/Slug is a downstream effector of various signaling pathways, including Akt, Wnt and SCF/ckit signaling.
Development References (4)
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Carpenter RL, Paw I, Dewhirst MW, Lo HW. Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene. 2014; ePub(Biology). View Reference
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Francí C, Takkunen M, Dave N, et al. Expression of Snail protein in tumor-stroma interface. Oncogene. 2006; 25(37):5134-5144. (Biology). View Reference
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Guo W, Keckesova Z, Donaher JL, et al. Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012; 148(5):1015-1028. (Biology). View Reference
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Hay ED, Zuk A. Transformations between epithelium and mesenchyme: normal, pathological, and experimentally induced. Am J Kidney Dis. 1995; 26(4):678-690. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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