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PE Mouse Anti-Human TCRαβ
PE Mouse Anti-Human TCRαβ

Two-color Flow Cytometric Analysis TCRαβ Expression on Human Peripheral Blood Lymphocytes

        

       Left Panel - Whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The cells were washed and then stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 340409) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Plot) or PE Mouse Anti-Human TCRαβ antibody (Cat. No. 564728; Right Plot). Two-color flow cytometric contour plots showing the coexpression of CD19 vs TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

       Right Panel - Erythrocyte-lysed whole blood was similarly washed and then stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human TCRαβ antibody (Right Plot). Two-color flow cytometric dot plots showing the coexpression of CD3 vs TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

       Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Two-color Flow Cytometric Analysis TCRαβ Expression on Human Peripheral Blood Lymphocytes

        

       Left Panel - Whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The cells were washed and then stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 340409) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Plot) or PE Mouse Anti-Human TCRαβ antibody (Cat. No. 564728; Right Plot). Two-color flow cytometric contour plots showing the coexpression of CD19 vs TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

       Right Panel - Erythrocyte-lysed whole blood was similarly washed and then stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human TCRαβ antibody (Right Plot). Two-color flow cytometric dot plots showing the coexpression of CD3 vs TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

       Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
alpha-beta T cell receptor, TCRAB, TRA@/TRB@; IP26A
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human T Cell Clone
Flow cytometry (Routinely Tested)
5 µl
AB_2738921
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  7. This product is sold under license. Purchase of this product does not include rights to (i) incorporate this product into the purchaser's own products for resale to end-users, or (ii) use this product to conduct for-profit research for or on behalf of another party. For information on obtaining a license to this product for such prohibited uses, contact INSERM, 7 rue Watt, 75013 Paris. Telephone: +33 1 55 03 01 60. Facsimile: +33 1 55 03 01 18. Email: techtransfert@inserm-transfert.fr
564728 Rev. 1
Antibody Details
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IP26

The IP26 monoclonal antibody specifically recognizes a monomorphic determinant on the αβ T-cell receptor (TCRαβ). The TCRαβ is a disulfide-linked 80 kDa heterodimer consisting of a 44 kDa α chain and a 37 kDa β chain. The TCRαβ is normally expressed on 50-70% of thymocytes and on a large fraction of mature T cells including greater than 95% of peripheral blood CD3+ T cells. The TCRαβ serves as a receptor for peptide antigens bound to MHC molecules. The IP26 antibody is mitogenic and useful for the flow cytometric analysis of TCRαβ+ T-cell subsets.

564728 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564728 Rev.1
Citations & References
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Development References (5)

  1. Agrawal SG, Marquet J, Plumas J, et al. Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue. Int Immunol. 2001; 13(4):441-450. (Clone-specific: Activation, Functional assay, Stimulation). View Reference
  2. Nikolova M, Marie-Cardine A, Boumsell L, Bensussan A. BY55/CD160 acts as a co-receptor in TCR signal transduction of a human circulating cytotoxic effector T lymphocyte subset lacking CD28 expression. Int Immunol. 2002; 14(5):445-451. (Clone-specific: Flow cytometry). View Reference
  3. Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). View Reference
  4. Ortonne N, Huet D, Gaudez C, et al. Significance of circulating T-cell clones in Sezary syndrome. Blood. 2006; 107(10):4030-4038. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  5. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Clone-specific: Flow cytometry). View Reference
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564728 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.