FITC Mouse Anti-Human CD61

This is a suggested alternate SKU for 565123. Contact us for additional support.

BD Pharmingen™ FITC Mouse Anti-Human CD61

Name: FITC Mouse Anti-Human CD61
Brand: BD Pharmingen™
SKU: 555753

Clone VI-PL2 (also known as VIPL2)

(RUO)

Flow cytometric analysis of CD61 expression on human peripheral blood platelets. Platelets were stained with either FITC Mouse Anti-Human CD61 (Cat. No. 555753/561913/557291; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™ system.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3

Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Cow (Tested in Development)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: III 866; IV P83; V T-124

Entrez Gene ID: 3690

RRID: AB_396094

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

FITC Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555748

FITC Mouse Anti-Human CD61 RUO

Size 25 Tests Cat No. 561913

FITC Mouse Anti-Human CD61 RUO

Size 50 Tests Cat No. 557291

555753 Rev. 7

Antibody Details

VI-PL2

The VI-PL2 monoclonal antibody specifically binds to CD61. CD61 is a 105 kDa transmembrane glycoprotein that is also known as integrin β3 and platelet glycoprotein IIIa (GPIIIa or GP3A). It is expressed on platelets, megakaryocytes, osteoclasts and endothelia. Integrin β3 associates with gpIIa (CD41) to form the CD41/CD61 complex which mediates platelet adhesion and aggregation. CD61 also associates with CD51 to form the CD51/CD61 complex (vitronectin receptor). CD61 appears to bind to fibrinogen, fibronectin, vWF, vitronectin, and thrombospondin to mediate cell adhesion.

555753 Rev. 7

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

555753 Rev.7

Citations & References

View product citations for antibody "555753" on CiteAb

Development References (1)

Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

555753 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.