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    FITC Mouse Anti-human CD106
    FITC Mouse Anti-human CD106
    Flow cytometric analysis of CD106 on TNF-α-activated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20ng/ml), then stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-human CD106 (Cat. No. 551146; solid line histogram). Fluorescent histograms were derived from gated events based on the light scattering characteristics for viable HUVEC cells.
    FITC Mouse Anti-human CD106
    Flow cytometric analysis of CD106 on TNF-α-activated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20ng/ml), then stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-human CD106 (Cat. No. 551146; solid line histogram). Fluorescent histograms were derived from gated events based on the light scattering characteristics for viable HUVEC cells.
    Product Details
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    BD Pharmingen™
    VCAM-1; Vascular cell adhesion protein 1; INCAM-100; L1CAM
    Human (QC Testing)
    Mouse IgG1, κ
    Human VCAM-1 Recombinant Protein
    Flow cytometry (Routinely Tested)
    20 µl
    V E112
    7412
    AB_394073
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    551146 Rev. 6
    Antibody Details
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    51-10C9

    The 51-10C9 monoclonal antibody specifically binds to CD106. CD106 is a 100-110 kDa type I transmembrane sialoglycoprotrein that is also known as Vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. CD106 is expressed at high levels on the surface of cytokine-stimulated endothelium, and at minimal levels on unstimulated endothelium. VCAM-1 serves as a ligand for the leukocyte integrins α4β1 (CD49d/CD29 complex; VLA-4) and α4β7 (LPAM-1). The 51-10C9 monoclonal antibody inhibits the in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.

    551146 Rev. 6
    Format Details
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    FITC
    Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    FITC
    Blue 488 nm
    494 nm
    518 nm
    551146 Rev.6
    Citations & References
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    View product citations for antibody "551146" on CiteAb

    Development References (4)

    1. Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA Jr. Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci U S A. 1987; 84(24):9238-9242. (Biology). View Reference
    2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
    3. Taichman DB, Cybulsky MI, Djaffar I, et al. Tumor cell surface alpha 4 beta 1 integrin mediates adhesion to vascular endothelium: demonstration of an interaction with the N-terminal domains of INCAM-110/VCAM-1. Cell Regul. 1991; 2(5):347-355. (Biology). View Reference
    4. van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88(6):2358-2361. (Immunogen). View Reference
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    551146 Rev. 6

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.