The 492621 monoclonal antibody specifically recognizes Platelet endothelial aggregation receptor 1 (PEAR1) which is also known as multiple EGF-like-domains 12 (MEGF12). PEAR1 is a ~150 kDa type I transmembrane glycoprotein and member of the MEGF family of proteins. The extracellular region contains of 15 EGF-like repeats whereas the cytoplasmic region contains five potential Src homology 3-binding, proline-rich domains. PEAR1 is expressed on platelets and endothelial cells. Upon platelet-platelet contact, PEAR1 signaling can stabilize platelet thrombi.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.