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    BV510 Rat Anti-Mouse Ly-6G
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    This product is the replacement for 740157.
    BV510 Rat Anti-Mouse Ly-6G
    Flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells.  BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; dashed line histogram) or BD Horizon™ BV510 Rat Anti-Mouse Ly-6G antibody (Cat. No. 569685; solid line histogram) at 1.0 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
    BV510 Rat Anti-Mouse Ly-6G
    Multiparameter flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells.  BALB/c Mouse bone marrow cells were stained with either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Plot) or BD Horizon™ BV510 Rat Anti-Mouse Ly-6G antibody (Cat. No. 569685; Right Plot) at 1.0 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
    BV510 Rat Anti-Mouse Ly-6G BV510 Rat Anti-Mouse Ly-6G
    Flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells.  BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; dashed line histogram) or BD Horizon™ BV510 Rat Anti-Mouse Ly-6G antibody (Cat. No. 569685; solid line histogram) at 1.0 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
    Multiparameter flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells.  BALB/c Mouse bone marrow cells were stained with either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Plot) or BD Horizon™ BV510 Rat Anti-Mouse Ly-6G antibody (Cat. No. 569685; Right Plot) at 1.0 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
    Product Details
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    BD Horizon™
    Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
    Mouse (QC Testing)
    Rat LEW, also known as Lewis IgG2a, κ
    Ly-6G-transfected EL4J Cell Line
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    546644
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

       BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

       For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    7. For U.S. patents that may apply, see bd.com/patents.
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    569685 Rev. 1
    Antibody Details
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    1A8

    The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

    569685 Rev. 1
    Format Details
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    BV510
    The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV510
    Violet 405 nm
    327 nm, 405 nm
    512 nm
    569685 Rev.1
    Citations & References
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    Development References (5)

    1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
    2. Fleming TJ, Malek TR. Multiple glycosylphosphatidylinositol-anchored Ly-6 molecules and transmembrane Ly-6E mediate inhibition of IL-2 production. J Immunol. 1994; 153(5):1955-1962. (Clone-specific: Flow cytometry, Functional assay, Inhibition). View Reference
    3. Fleming TJ, O'HUigin C, Malek TR. Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J Immunol. 1993; 150(12):5379-5390. (Biology). View Reference
    4. Han G, Havnaer A, Lee HH, Carmichael DJ, Martinez LR. Biological depletion of neutrophils attenuates pro-inflammatory markers and the development of the psoriatic phenotype in a murine model of psoriasis.. Clin Immunol. 2020; 210:108294. (Clone-specific: Blocking, Flow cytometry). View Reference
    5. Nowroozilarki N, Öz HH, Schroth C, et al. Anti-inflammatory role of CD11b+Ly6G+ neutrophilic cells in allergic airway inflammation in mice.. Immunol Lett. 2018; 204:67-74. (Clone-specific: Flow cytometry). View Reference
    View All (5) View Less
    569685 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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