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    BV510 Mouse Anti-Human CD43
    hCD43_BV510_overlay.png

    Flow cytometric analysis of CD43 expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV510 Mouse Anti-Human CD43 antibody (Cat. No. 563377; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

    hCD43_BV510_overlay.png Green-Cap_Violet.png

    Flow cytometric analysis of CD43 expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV510 Mouse Anti-Human CD43 antibody (Cat. No. 563377; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

    Product Details
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    BD Horizon™
    SPN; Sialophorin; Galactoglycoprotein/GALGP; GPL115; LEUK; Leukosialin; LSN
    Human (QC Testing)
    Mouse IgG1, κ
    Flow cytometry (Routinely Tested)
    5 µl
    VI N-L166
    AB_2722767
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Brilliant Violet™ 510 is a trademark of Sirigen.
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    563377 Rev. 1
    Antibody Details
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    1G10

    The 1G10 monoclonal antibody specifically binds to CD43, a 95-135 kDa sialoglycoprotein that is highly expressed on most human leucocytes. CD43 is encoded by the SPN gene and is also known as Sialophorin, Galactoglycoprotein (GALGP), Leukosialin, and Leukocyte sialoglycoprotein. The CD43 antigen is expressed on T cells, pre-B cells and activated B cells, NK cells and granulocytes, but is not present on resting peripheral blood B cells, red blood cells, platelets and non-hematopoietic cells. CD43 is enzymatically shed from leucocyte  surfaces following activation by various stimuli. CD43 appears to be involved in intercellular interactions that regulate T, B, and NK cell functions. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections with TUF pretreatment.

    The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

    563377 Rev. 1
    Format Details
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    BV510
    The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    BV510
    327 nm, 405 nm
    512 nm
    563377 Rev.1
    Citations & References
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    View product citations for antibody "563377" on CiteAb

    Development References (4)

    1. Bazil V, Strominger JL. CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes. Proc Natl Acad Sci U S A. 1993 May; 90(9):3792-3796. (Biology). View Reference
    2. Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987; 17(10):1523-1526. (Biology). View Reference
    3. Horejsi V, Stockinger H. CD43 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:494-497.
    4. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:111-112.
    View All (4) View Less
    563377 Rev. 1

    Please refer to Support Documents for Quality Certificates

     

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

     

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.