The site is undergoing maintenance. Ordering can continue through fax and phone.
If you encounter problems while ordering. Please contact us at BDBCustomerService@bd.com
See More
BD Horizon™ BV421 Mouse Anti-Human CD71
Clone M-A712 (RUO)
Flow cytometric analysis of CD71 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated (3 days) peripheral blood mononuclear cells were stained with either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human CD71 antibody (Cat. No. 562995; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD71 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated (3 days) peripheral blood mononuclear cells were stained with either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human CD71 antibody (Cat. No. 562995; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
TFR; TFRC; Trfr; TfR1; Transferrin receptor protein 1
Human (QC Testing)
Mouse BALB/c IgG2a, κ
T-CLL and Jurkat Cells
Flow cytometry (Routinely Tested)
5 µl
AB_2737939
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
BV421 Mouse IgG2a, k Isotype Control RUO
Size
50 µg
Cat No.
562439
562995 Rev. 1
Antibody Details
M-A712
The M-A712 monoclonal antibody specifically binds to CD71 which is also known as the transferrin receptor (TFR). This type II transmembrane glycoprotein is expressed on cells as a disulfide-linked homodimer comprised of 95 kDa monomers. CD71 is expressed on activated lymphocytes, monocytes, macrophages, erythroid progenitors, brain endothelium, and most proliferating cells. CD71 is not expressed on resting lymphocytes and is upregulated during lymphocyte responses to antigens or mitogens. Through an endocytic pathway, the transferrin receptor mediates cellular iron uptake by binding and internalizing iron that is bound to transferrin. After releasing iron within the low pH endosomal environment, transferrin and its receptor can be recycled to the cell surface.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
562995 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
562995 Rev.1
Citations & References
View product citations for antibody "562995" on CiteAb
Development References (4)
-
Makita S, Kanai T, Oshima S, et al. CD4+CD25bright T cells in human intestinal lamina propria as regulatory cells. J Immunol. 2004; 173(5):3119-3130. (Clone-specific: Flow cytometry). View Reference
-
Ni J, Hembrador E, Di Bisceglie AM, et al. Accumulation of B lymphocytes with a naive, resting phenotype in a subset of hepatitis C patients. J Immunol. 2003; 170(6):3429-3439. (Clone-specific: Flow cytometry). View Reference
-
Schwarting R, Stein H. Cluster report: CD71. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:455-460.
-
Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
562995 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.