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BV421 Mouse Anti-Human CD44v6
BV421 Mouse Anti-Human CD44v6

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD44v6 antibody (Cat. No. 749713) on human peripheral blood (right figure), with Isotype Control (left figure). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD44v6 antibody (Cat. No. 749713) on human peripheral blood (right figure), with Isotype Control (left figure). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

Product Details
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BD OptiBuild™
CD44v6; CD44 v6; CD44 containing exon v6
Human (Tested in Development)
Mouse IgG1, κ
Recombinant human CD44v3-10 Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2873967
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
749713 Rev. 4
Antibody Details
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2F10

The 2F10 monoclonal antibody specifically recognizes exon v6-containing isoforms of the CD44 adhesion receptor (CD44v6). CD44 is a widely-expressed, 85-250 kDa type I transmembrane glycoprotein that regulates cellular adhesion, growth, and migration and is involved in cell-cell interactions. CD44 is a member of the hyaladherin family of hyaluronan-binding proteins. It serves as a multifunctional receptor for hyaluronic acid and other ligands including collagens, fibronectin, matrix metalloproteinases, and osteopontin. The standard form of CD44 (CD44s) contains an extracellular region with a hyaluronan-binding link domain and a stem region devoid of variable exon sequences that are followed by a transmembrane domain and a cytoplasmic tail. The cytoplasmic region is linked to the cytoskeleton and may be involved in signaling interactions. Alternative splicing of CD44 gene transcripts results in the differential inclusion of 10 variant exons (v1-10) within the stem region, either individually or in multiple combinations. This results in a large number of variant CD44 (CD44v) isoforms with different cell surface expression levels and functions. CD44 isoforms containing exon v6 (CD44v6) are variably expressed on epithelial cells, monocytes, macrophages, dendritic cells, and Langerhans cells. It is expressed on certain tumor cells and on cancer stem cells (CSCs) and is implicated in tumor cell metastasis. In addition to playing roles in cellular adhesion and migration, CD44 isoforms with exon v6 sequences may serve as coreceptors for some growth factors and cytokines including hepatocyte growth factor (HGF) which is involved in driving c-Met signaling.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

749713 Rev. 4
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
749713 Rev.4
Citations & References
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Development References (5)

  1. Bouwer AL, Saunderson SC, Caldwell FJ, et al. NK cells are required for dendritic cell-based immunotherapy at the time of tumor challenge.. J Immunol. 2014; 192(5):2514-21. (Clone-specific: Flow cytometry). View Reference
  2. P. A. Gorer, S. Lyman, G. D. Snell. Studies on the Genetic and Antigenic Basis of Tumour Transplantation. Linkage between a Histocompatibility Gene and 'Fused' in Mice. Proc R Soc Lond B Biol Sci. 1948; 135(881):499-505. (Biology).
  3. Springer TA. Cell-surface differentiation in the mouse. Characterization of "jumping" and "lineage" antigens using xenogeneic rat monoclonal antibodies. In: Kennett RH, McKearn TJ, Bechtol KB, ed. Monoclonal antibodies. Hybridomas: A new dimension in biological analyses. New York and London: Plenum Press; 1980:185-217.
  4. Stallcup KC, Springer TA, Mescher MF. Characterization of an anti-H-2 monoclonal antibody and its use in large-scale antigen purification. J Immunol. 1981; 127(30):923-930. (Immunogen: Flow cytometry, Immunoaffinity chromatography, Immunoprecipitation, Radioimmunoassay). View Reference
  5. Watanabe Y, Kuribayashi K, Miyatake S, et al. Exogenous expression of mouse interferon gamma cDNA in mouse neuroblastoma C1300 cells results in reduced tumorigenicity by augmented anti-tumor immunity.. Proc Natl Acad Sci USA. 1989; 86(23):9456-60. (Clone-specific: Flow cytometry). View Reference
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749713 Rev. 4

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.