The VIM15 monoclonal antibody specifically binds to CD92 which is also known as Solute carrier family 44 member 1 (SLC44A1), or Choline transporter-like protein 1 (CHTL1). CD92 is a multipass membrane glycoprotein that belongs to the choline transporter-like family. It contains a tyrosine-based inhibitory motif (ITIM) in its cytoplasmic C-terminus. CD92 appears to be involved in choline transport for cell membrane phospholipid synthesis and in regulatory cell-signaling pathways. CD92 is strongly expressed by monocytes and at lower levels by granulocytes and some dendritic cells. It is generally expressed at low levels by B cells, endothelial cells, epithelial cells, and subsets of T cells, or NK cells. The VIM15 antibody has been shown to augment lipopolysaccharide-induced IL-10 production by monocyte-derived dendritic cells (Mo-DC). When cultured with ionomycin or calcium ionophore, maturing Mo-DC showed downregulation of CD92 expression.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).