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BUV563 Mouse Anti-Human CD11a
Product Details
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BD OptiBuild™
LFA-1α; Lymphocyte (Leukocyte) function-associated antigen 1 α chain; ITGAL
Human (Tested in Development)
Mouse IgG2a
Flow cytometry (Qualified)
0.2 mg/ml
AB_2873236
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV563 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
748833 Rev. 2
Antibody Details
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G25.2

The G-25.2 monoclonal antibody specifically recognizes CD11a which is also known as Leukocyte function-associated molecule 1 alpha chain (LFA-1A or LFA-1α). CD11a is a ~180 kDa type I transmembrane glycoprotein that is encoded by ITGAL (Integrin subunit alpha L) that belongs to integrin α-chain family. CD11a is a leucocyte adhesion receptor that noncovalently complexes with CD18 to form the LFA-1 heterodimer (CD11a/CD18) which binds to intercellular adhesion molecules (ICAMs) expressed on target tissues including ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICAM-4, or ICAM-5. The CD11a antigen is expressed on virtually all normal peripheral blood leucocytes. It is essential for many immune responses that require cell-to-cell contact, such as lymphocyte adhesion, natural killer (NK) and T-lymphocyte cytolysis, and T lymphocyte proliferation. Activation of lymphocytes through the T-cell antigen receptor (TCR) upregulates LFA-1 function, converting the antigen to a high-avidity state. Expression of LFA-1 on monocytes is upregulated by γ-interferon (IFN-γ). While normal cells possess both the α and β LFA-1 chains, cells of subjects with some leukemias, lymphomas, or leucocyte adhesion deficiency (LAD) may lack the entire LFA-1 molecule or the α or β chain. The absence of LFA-1 on tumor cells may provide a mechanism for their escape from immune surveillance.

The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

748833 Rev. 2
Format Details
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BUV563
The BD Horizon Brilliant™ Ultraviolet 563 (BUV563) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 564-nm. BUV563, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 560-nm (e.g., a 560/40 or a 585/15-nm bandpass filter). The acceptor dye can be excited by the Blue (488-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV563
Ultraviolet 355 nm
350 nm
564 nm
748833 Rev.2
Citations & References
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Development References (6)

  1. Anderson DC, Springer TA. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.. Annu Rev Med. 1987; 38:175-94. (Biology). View Reference
  2. Clayberger C, Wright A, Medeiros LJ, et al. Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance.. Lancet. 1987; 2(8558):533-6. (Biology). View Reference
  3. Fischer A, Griscelli C, Blanche S, et al. Prevention of graft failure by an anti-HLFA-1 monoclonal antibody in HLA-mismatched bone-marrow transplantation.. Lancet. 1986; 2(8515):1058-61. (Biology). View Reference
  4. Inghirami G, Wieczorek R, Zhu BY, Silber R, Dalla-Favera R, Knowles DM. Differential expression of LFA-1 molecules in non-Hodgkin's lymphoma and lymphoid leukemia. Blood. 1988; 72(4):1431-1434. (Biology). View Reference
  5. Mentzer SJ, Faller DV, Burakoff SJ. Interferon-gamma induction of LFA-1-mediated homotypic adhesion of human monocytes.. J Immunol. 1986; 137(1):108-13. (Biology). View Reference
  6. Uciechowski P, Schmidt RE. Cluster report: CD11. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:543.
View All (6) View Less
748833 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.