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    Biotin Mouse Anti-Mouse IgG2a[a]
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    BD Pharmingen™
    Igh-1a
    Mouse (QC Testing)
    Mouse SJL IgG2a, κ
    Not reported
    ELISA (Routinely Tested), Flow cytometry (Tested During Development)
    0.5 mg/ml
    AB_394887
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

    Biotinylated 8.3 mAb may be used as a secondary reagent in immunofluorescent staining with flow cytometric analysis. Note: The isotype of clone 8.3  is mouse (SJL) IgG2a[b], κ.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    553502 Rev. 17
    Antibody Details
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    8.3

    The 8.3 antibody reacts specifically with mouse IgG2a of Igh-Ca and the d, e, f, g, h, j, n, and o haplotypes (eg, BALB/c, C58, and A, AKR, CBA, C3H/He, NZB). It does not react with IgG2a of Igh-Cb nor the c and p haplotypes (eg, C57BL/6, C57BL/10, SJL, DBA/1, DBA/2, SWR).

    553502 Rev. 17
    Format Details
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    Biotin
    Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
    Biotin
    553502 Rev.17
    Citations & References
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    View product citations for antibody "553502" on CiteAb

    Development References (2)

    1. Huang CM, Parsons M, Oi VT, Huang HJ, Herzenberg LA. Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):311-321. (Clone-specific). View Reference
    2. Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Clone-specific). View Reference
    553502 Rev. 17

     

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.