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Biotin Mouse Anti-Human CD326 (EpCAM)
Biotin Mouse Anti-Human CD326 (EpCAM)
Flow cytometric analysis of CD326 (EpCAM) expression on Human colorectal adenocarcinoma cell line. Cells from the Human HT-29 (Colorectal Carcinoma, ATCC HTB-38) cell line were stained with Biotin Mouse IgG2b, κ Isotype Control (Cat. No. 555741; dashed line histogram) or Biotin Mouse Anti-Human CD326 (EpCAM) antibody (Cat. No. 567937; solid line histogram) followed by PE Streptavidin (Cat. No. 554061). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The histogram showing CD326 (EpCAM) expression [or Ig Isotype control staining] was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of CD326 (EpCAM) expression on Human colorectal adenocarcinoma cell line. Cells from the Human HT-29 (Colorectal Carcinoma, ATCC HTB-38) cell line were stained with Biotin Mouse IgG2b, κ Isotype Control (Cat. No. 555741; dashed line histogram) or Biotin Mouse Anti-Human CD326 (EpCAM) antibody (Cat. No. 567937; solid line histogram) followed by PE Streptavidin (Cat. No. 554061). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The histogram showing CD326 (EpCAM) expression [or Ig Isotype control staining] was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1; adenocarcinoma-associated antigen
Human (QC Testing)
Mouse BALB/c IgG2b, κ
Human breast carcinoma Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
VIII 80168
4072
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
567937 Rev. 2
Antibody Details
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9C4

The 9C4 monoclonal antibody specifically binds to human epithelial cell adhesion molecule (EpCAM), also known as adenocarcinoma associated antigen and CD326. EpCAM is an approximately 40-kDa type 1 transmembrane glycoprotein and adhesion molecule that mediates intercellular interactions via homotypic adhesion. The epithelial cells present in non-squamous epithelia and tumors derived from such cells show EpCAM expression. Tumors arising from non-epithelial cells, such as lymphoma, mesothelioma, neuroblastoma, and melanoma, do not express EpCAM. The normal epithelial cells reactive with anti-EpCAM antibodies are those present in the (lower) respiratory tract; the (lower) gastrointestinal tract; tubules in the kidney; the surface epithelium of the ovary; the exocrine and endocrine pancreas; secondary germ cells of telogenic hair follicles; and secretory tubules of sweat glands in the skin, whereas the epidermis is negative. In addition, all epithelial cells in the thyroid and epithelial cells in the thymus show EpCAM expression, while the outer cortex and Hassall's corpuscles have low expression. EpCAM is expressed on a variety of stem and progenitor cells, and its down-regulation is associated with decreased proliferation and differentiation toward endoderm and mesoderm lineages.

567937 Rev. 2
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
567937 Rev.2
Citations & References
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View product citations for antibody "567937" on CiteAb

Development References (5)

  1. Bühring HJ, Müller T, Herbst R, et al. The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages.. Leukemia. 1996; 10(1):106-16. (Clone-specific: Flow cytometry). View Reference
  2. Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W, Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial cellular adhesion molecule, a cell surface antigen expressed in early steps of erythropoiesis.. Exp Hematol. 2002; 30(6):537-45. (Clone-specific: Flow cytometry). View Reference
  3. Ng VY, Ang SN, Chan JX, Choo AB. Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells. Stem Cells. 2010; 28(1):29-35. (Biology). View Reference
  4. Patriarca C, Macchi RM, Marschner AK, Mellstedt H. Epithelial cell adhesion molecule expression (CD326) in cancer: a short review. Cancer Treat Rev. 2012; 38(1):68-75. (Biology). View Reference
  5. Trzpis M, McLaughlin PM, de Leij LM, Harmsen MC. Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule. Am J Pathol. 2007; 171(2):386-395. (Biology: Bioassay). View Reference
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567937 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.