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    Alexa Fluor® 700 Hamster Anti-Mouse TCR β Chain
    560705Image1.png

    Flow cytometric analysis of the T-cell receptor (TcR) β-chain on mouse splenocytes.  Left Panel: Splenocytes from C57BL/6 mice were stained with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (shaded) or with the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (unshaded).  Middle and Right Panels: Splenocytes from C57BL/6 mice were stained with both a PE Rat Anti-Mouse CD4 antibody (Cat.No. 553049) and a PE Rat Anti-Mouse CD8a antibody (Cat.No. 553033) in conjunction with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (middle panel) or the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (right panel). Histograms and dot plots were derived from gated events based on light scattering characteristics for splenocytes.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

    560705_IMAGE3.png

    Flow cytometric analysis of the T-cell receptor (TcR) β-chain on mouse splenocytes.  Left Panel: Splenocytes from C57BL/6 mice were stained with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (shaded) or with the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (unshaded).  Middle and Right Panels: Splenocytes from C57BL/6 mice were stained with both a PE Rat Anti-Mouse CD4 antibody (Cat.No. 553049) and a PE Rat Anti-Mouse CD8a antibody (Cat.No. 553033) in conjunction with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (middle panel) or the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (right panel). Histograms and dot plots were derived from gated events based on light scattering characteristics for splenocytes.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

    560705Image1.png 560705_IMAGE3.png

    Flow cytometric analysis of the T-cell receptor (TcR) β-chain on mouse splenocytes.  Left Panel: Splenocytes from C57BL/6 mice were stained with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (shaded) or with the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (unshaded).  Middle and Right Panels: Splenocytes from C57BL/6 mice were stained with both a PE Rat Anti-Mouse CD4 antibody (Cat.No. 553049) and a PE Rat Anti-Mouse CD8a antibody (Cat.No. 553033) in conjunction with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (middle panel) or the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (right panel). Histograms and dot plots were derived from gated events based on light scattering characteristics for splenocytes.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

    Flow cytometric analysis of the T-cell receptor (TcR) β-chain on mouse splenocytes.  Left Panel: Splenocytes from C57BL/6 mice were stained with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (shaded) or with the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (unshaded).  Middle and Right Panels: Splenocytes from C57BL/6 mice were stained with both a PE Rat Anti-Mouse CD4 antibody (Cat.No. 553049) and a PE Rat Anti-Mouse CD8a antibody (Cat.No. 553033) in conjunction with either a Alexa Fluor® 700 Hamster IgG2, λ1 isotype control (middle panel) or the Alexa Fluor® 700 Hamster Anti-Mouse TcR β-chain antibody (right panel). Histograms and dot plots were derived from gated events based on light scattering characteristics for splenocytes.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

    Product Details
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    BD Pharmingen™
    Mouse (QC Testing)
    Armenian Hamster IgG2, λ1
    TCR affinity-purified from mouse T-cell hybridoma DO-11.10
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_1727573
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
    4. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
    5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
    7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    560705 Rev. 1
    Antibody Details
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    H57-597

    The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

    560705 Rev. 1
    Format Details
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    Alexa Fluor™ 700
    Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    Alexa Fluor™ 700
    697 nm
    719 nm
    560705 Rev.1
    Citations & References
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    View product citations for antibody "560705" on CiteAb

    Development References (3)

    1. Gascoigne NR. Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990; 265(16):9296-9301. (Clone-specific: Immunofluorescence, Immunoprecipitation). View Reference
    2. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation, Functional assay, Stimulation). View Reference
    3. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Immunogen: Activation, Flow cytometry, Functional assay, Stimulation). View Reference
    560705 Rev. 1

    Please refer to Support Documents for Quality Certificates

     

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

     

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.