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558156_image1.png

Flow cytometric analysis of CD205 expression on human monocyte-derived cultured dendritic cells. Dendritic cells were stained with either Alexa Fluor® 647 Mouse Anti-Human CD205 (Cat. No. 558156; solid line histogram) or Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557732; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable dendritic cells.

558156_image1.png

Flow cytometric analysis of CD205 expression on human monocyte-derived cultured dendritic cells. Dendritic cells were stained with either Alexa Fluor® 647 Mouse Anti-Human CD205 (Cat. No. 558156; solid line histogram) or Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557732; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable dendritic cells.

558156_image1.png 558156_image1.png Red-Cap-Red-Label.jpg

Flow cytometric analysis of CD205 expression on human monocyte-derived cultured dendritic cells. Dendritic cells were stained with either Alexa Fluor® 647 Mouse Anti-Human CD205 (Cat. No. 558156; solid line histogram) or Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557732; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable dendritic cells.

Flow cytometric analysis of CD205 expression on human monocyte-derived cultured dendritic cells. Dendritic cells were stained with either Alexa Fluor® 647 Mouse Anti-Human CD205 (Cat. No. 558156; solid line histogram) or Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557732; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable dendritic cells.

Product Details
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BD Pharmingen™
DEC-205; Lymphocyte Antigen 75; LY75; Ly-75; CLEC13B; GP200-MR6
Human (QC Testing)
Mouse IgG1, κ
Recombinant Human DEC-205-Ig Proteins
Flow cytometry (Routinely Tested)
0.2 mg/ml
VII
AB_397054
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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558156 Rev. 4
Antibody Details
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MMRI-7

The MMRI-7 monoclonal antibody specifically recognizes CD205 which is also known as DEC-205, Lymphocyte antigen 75 (Ly-75), or C-type lectin domain family 13 member B (CLEC13B). CD205 is a ~205 kDa type I transmembrane glycoprotein with C-type lectin external domains. DEC-205 is expressed on myeloid and plasmacytoid dendritic cells, thymic epithelial cells, and at lower levels on monocytes, T cells, B cells, and natural killer (NK) cells. DEC-205 likely functions as an endocytic receptor and is involved in antigen uptake and processing.

558156 Rev. 4
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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Alexa Fluor™ 647
653 nm
669 nm
558156 Rev.4
Citations & References
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View product citations for antibody "558156" on CiteAb

Development References (5)

  1. Guo M, Gong S, Maric S, et al. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 August; 61(8):729-738. (Biology). View Reference
  2. Kato M, Khan S, Gonzalez N, et al. Hodgkin's lymphoma cell lines express a fusion protein encoded by intergenically spliced mRNA for the multilectin receptor DEC-205 (CD205) and a novel C-type lectin receptor DCL-1.. J Biol Chem. 2003; 278(36):34035-41. (Clone-specific). View Reference
  3. Kato M, MacDonald K, Munster D, Clark GJ, Hart DNJ. CD205 (DEC-205) Workshop Panel report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:298-300.
  4. Kato M, Neil TK, Clark GJ, Morris CM, Sorg RV, Hart DN. cDNA cloning of human DEC-205, a putative antigen-uptake receptor on dendritic cells. Immunogenetics. 1998; 47(6):442-450. (Biology). View Reference
  5. Kato M, Neil TK, Fearnley DB, McLellan AD, Vuckovic S, Hart DN. Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells. Int Immunol. 2000; 12(11):1511-1519. (Biology). View Reference
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558156 Rev. 4

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.