Name: Alexa Fluor® 647 Mouse Anti-A2B5
Brand: BD Pharmingen™
SKU: 563776

Alexa Fluor® 647 Mouse Anti-A2B5

Clone 105/A2B5 (also known as antibody A2B5, F12A2B5; 105)

(RUO)

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Protocol Library

Scientific Resources

Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506; left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806, dashed line histograms) or Alexa Fluor® 647 Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing), Human (Tested in Development), Rat, Chicken (Reported)

Isotype: Mouse BALB/c IgM

Immunogen: Chicken embryo retina Cells

Application: Flow cytometry (Routinely Tested), Bioimaging, Immunofluorescence (Not Recommended)

Vol. Per Test: 5 µl

RRID: AB_2738422

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Alexa Fluor® 647 Mouse IgM, κ Isotype Control RUO

Size 0.1 mg Cat No. 560806

Accutase™ Cell Detachment Solution RUO

Size 100 mL Cat No. 561527

563776 Rev. 1

Antibody Details

105/A2B5

The 105/A2B5 monoclonal antibody binds to gangliosides in neural tissue in the retina, brain, spinal cord, and dorsal root ganglia. The antigenic gangliosides are found on the plasma membrane of neural cell bodies, but not axons or dendrites. Expression levels of the gangliosides that mAb 105/A2B5 binds to, including GT3 and its O-acetylated derivative, decrease during embryonic development of the brain. Thus, mAb 105/A2B5 may be used to monitor the maturation of brain tissue. A2B5 antigens have also been associated with numerous tumors, including glioblastoma multiforme tumor cells, pancreatic islet tumor cells, and rat insulinoma cells, and are thought to contribute to chemoresistance and increased tumor proliferation and recurrence.

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563776 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

563776 Rev.1

Citations & References

Development References (2)

Balik V, Mirossay P, Bohus P, Sulla I, Mirossay L, Sarissky M. Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy. 2009; 29:845-858. (Clone-specific: Flow cytometry). View Reference
Eisenbarth GS, Walsh FS, Nirenberg M. Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci U S A. 1979; 76(10):4913-4917. (Immunogen: Cytotoxicity, Immunofluorescence). View Reference

563776 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.