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Purified Mouse Anti-Human CD15
Product Details
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Mouse BALB/c IgM, κ
U-937 histiocytic cell line
Flow cytometry
50 μg/mL
20 μL
HEPES buffer with gelatin and 0.1% sodium azide.

Preparation And Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347420 Rev. 1
Antibody Details
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The CD15 antibody, clone MMA, is derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the U-937 histiocytic cell line.

CD15 recognizes a human myelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.

347420 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (4)

  1. Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982 May; 23(2):172-188. (Biology). View Reference
  2. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984; 82:29. (Biology).
  3. Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed-Sternberg cells in Hodgkin's disease: an immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985; 119:244. (Biology).
  4. Skubitz K, Balke J, Ball E, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:800-805.
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 


Although not required, these products are manufactured in accordance with Good Manufacturing Practices.