.png)
Preparation And Storage
The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Anti-Hu–IL-8, clone AS14, is derived from the hybridization of mouse P3X63Ag8 cells with spleen cells from BALB/c mice immunized with recombinant human IL-8. Anti-Human Interleukin-8 (Anti-Hu–IL-8) recognizes a nonglycosylated protein of approximately 8 kilodaltons (kd).
Development References (11)
-
Cassatella MA, Meda L, Bonora S, et al. Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1 β in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med. 1993; 178:2207-2211. (Biology).
-
DeForge LE, Kenney JS, Jones ML, et al. Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. Separation of LPS- and cytokine-stimulated components using anti-tumor necrosis factor and anti-IL-1 antibodies. J Immunol. 1992; 148:2133-2141. (Biology).
-
Francia di Celle P, Carbone A, Marchis D, et al. Cytokine gene expression in B-chronic lymphocytic leukemia: Evidence of constitutive interleukin-8 (IL-8) mRNA expression and secretion of biologically active IL-8 protein. Blood. 1994; 84:220-228. (Biology).
-
Fujishima S, Nakamura H, Yasuhiro W, et al. Cell-associated IL-8 in human blood monocytes: Analysis by flow cytometry. Cytometry. 1996; 24:382-389. (Biology).
-
Hebert CA, Luscinskas FW, Kiely JM, et al. Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils. J Immunol. 1990; 145(9):3033-3040. (Biology). View Reference
-
Kunkel SL, Strieter RM, Lindley IJD. Chemokines: new ligands, receptors and activities. Immunol Today. 1995; 16:559-561. (Biology).
-
Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989; 243(4897):1464-1466. (Biology). View Reference
-
Leonard EJ, Skeel A, Yoshimura T, Noer K, Kutvirt S, Van Epps D. Leukocyte specificity and binding of human neutrophil attractant/activation protein-1. J Immunol. 1990; 144(41323):1323-1330. (Biology). View Reference
-
Oppenheim JJ, Zachariae COC, Mukaida N, et al. Properties of the novel proinflammatory supergene intercrine cytokine family. Ann Rev Immunol. 1991; 9:617-648. (Biology).
-
Uguccioni M, Meliconi R, Nesci S, et al. Elevated interleukin-8 serum concentrations in β -thalassemia and graft-versus-host disease. Blood. 1993; 81:2252-2256. (Biology).
-
Yoshimura T, Matsushima K, Oppenheim JJ, et al. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1(IL-1). J Immunol. 1987; 139:788-793. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.