FITC Mouse Anti-Human CD7

BD™ FITC Mouse Anti-Human CD7

Name: FITC Mouse Anti-Human CD7
Brand: BD™
SKU: 347483

Clone 4H9 (also known as Leu-9)

(RUO (GMP))

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Product Details

Brand: BD™

Reactivity: Human

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: T-acute lymphoblastic leukemia cells

Application: Flow cytometry

Concentration: 30 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 924

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The monoclonal antibody is supplied as 60 µg purified immunoglobulin in 2.0 mL (30 µg/mL) of phosphate-buffered saline.The FITC conjugate is supplied as 60 µg in 2.0 mL (30 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

4H9

CD7, clone 4H9, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with T-acute lymphoblastic leukemia cells.

CD7 recognizes a human T- and natural killer (NK)–lymphocyte antigen, Mr 40 kilodaltons (kd).

347483 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

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Citations & References

Development References (7)

Foon KA, Todd RF. Immunologic classification of leukemia and lymphoma.. Blood. 1986; 68(1):1-31. (Biology). View Reference
Link M, Warnke R, Finlay J, et al. A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. Blood. 1983; 62:722-728. (Biology).
Palker TJ, Scearce RM, Hensley LL, Ho W, Haynes BF. Comparison of the CD7 (3A1) group of T cell workshop antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II. Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:303-313.
Picker LF, Weiss LM, Medeiros LJ, Wood GS, Warnke RA. Immunophenotypic criteria for the diagnosis of non-Hodgkin’s lymphoma. Am J Pathol. 1987; 128:181. (Biology).
Weiss LM, Crabtree GS, Rouse RV, Warnke RA. Morphologic and immunologic characterization of 50 peripheral T-cell lymphomas. Am J Pathol. 1985; 118:316-324. (Biology).
Weiss LM, Wood GS, Warnke RA. Immunophenotypic differences between dermatopathic lymphadenopathy and lymph node involvement in mycosis fungoides. Am J Pathol. 1985; 120:179-185. (Biology).
Wood GS, Abel EA, Hoppe RT, Warnke RA. Leu-8 and Leu-9 antigen phenotypes: immunologic criteria for the distinction of mycosis fungoides from cutaneous inflammation. J Am Acad Dermatol. 1986; 14:1006-1013. (Biology).

View All (7)

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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