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FITC Mouse Anti-Human CD27
Product Details
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BD™
TNFRSF7; Tumor necrosis factor receptor superfamily, member 7; Tp55; S152
Human
Mouse BALB/c IgG1
Human Activated Peripheral Blood Cells
Flow cytometry
0.8 μg/mL
20 μL
VI T6T037
939
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340424 Rev. 1
Antibody Details
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L128

The CD27 antibody, clone L128, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with activated peripheral blood lymphocytes.

The CD27 antibody recognizes a 110–120-kilodalton (kDa) disulfide-linked homodimer comprised of two 55-kDa polypeptide chains. The CD27 antigen is a lymphocyte-specific member of the tumor necrosis factor receptor (TNFR) super family, which also includes nerve growth factor receptor, CD30, CD40, CD95 (Fas), CD120a, CD120b, CD134 (OX 40), and CD137. The CD27 antigen is also known as S152, T14, TNFRSF7, and Tp55.

340424 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
340424 Rev.1
Citations & References
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Development References (30)

  1. Akiba H, Nakano H, Nishinaka S, et al. CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-κB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-κB-inducing kinase. J Biol Chem. 1998; 273:13353-13358. (Biology).
  2. Appay V, Papagno L, Spina CA, et al. Dynamics of T cell responses in HIV infection. J Immunol. 2002; 168:3660-3666. (Biology).
  3. Baars PA, Maurice MM, Rep M, Hooibrink B, van Lier RA. Heterogeneity of the circulating human CD4+ T cell population. Further evidence that the CD4+CD45RA-CD27- T cell subset contains specialized primed T cells. J Immunol. 1995; 154(1):17-25. (Biology). View Reference
  4. Bowman MR, Crimmins MA, Yetz-Aldape J, Kriz R, Kelleher K, Herrmann S. The cloning of CD70 and its identification as the ligand for CD27. J Immunol. 1994; 152(4):1756-1761. (Biology). View Reference
  5. Camerini D, Walz G, Loenen WA, Borst J, Seed B. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. J Immunol. 1991; 147(9):3165-3169. (Biology). View Reference
  6. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  7. Clinical and Laboratory Standards Institute. 2005. (Biology).
  8. De Jong R, Brouwer M, Hooibrink B, Van der Pouw-Kraan T, Miedema F, Van Lier RA. The CD27- subset of peripheral blood memory CD4+ lymphocytes contains functionally differentiated T lymphocytes that develop by persistent antigenic stimulation in vivo. Eur J Immunol. 1992; 22(4):993-999. (Biology). View Reference
  9. Elrefaei M, El-Sheikh N, Kamal K, Cao H. HCV-specific CD27-CD28- memory T cells are depleted in hepatitis C virus and Schistosoma mansoni co-infection. Immunology. 2003; 110:513-518. (Biology).
  10. Elson LH, Shaw S, Van Lier RA, Nutman TB. T cell subpopulation phenotypes in filarial infections: CD27 negativity defines a population greatly enriched for Th2 cells. Int Immunol. 1994; 6:1003-1009. (Biology).
  11. Gruss HJ, Dower SK. Tumor necrosis factor ligand superfamily: involvement in the pathology of malignant lymphomas. Blood. 1995; 85:3378-3404. (Biology).
  12. He L, Fox MH. Comparison of flow cytometry and western blotting to measure Hsp70. Cytometry. 1996; 25:280-286. (Biology).
  13. Hintzen RQ, De Jong R, Lens SM, Brouwer M, Baars P, Van Lier RA. Regulation of CD27 expression on subsets of mature T lymphocytes. J Immunol. 1993; 151:2426-2435. (Biology).
  14. Hintzen RQ, Lens SM, Beckmann MP, Goodwin RG, Lynch D, van Lier RA. Characterization of the human CD27 ligand, a novel member of the TNF gene family. J Immunol. 1994; 152(4):1762-1773. (Biology). View Reference
  15. Hintzen RQ, de Jong R, Hack CE, et al. A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex. J Immunol. 1991; 147(1):29-35. (Biology). View Reference
  16. Kobata T, Agematsu K, Kameoka J, Schlossman SF, Morimoto C. CD27 is a signal-transducing molecule involved in CD45RA+ naive T cell costimulation. J Immunol. 1994; 153(12):5422-5432. (Biology). View Reference
  17. Kobata T, Jacquot S, Kozlowski S, Agematsu K, Schlossman SF, Morimoto C. CD27-CD70 interactions regulate B-cell activation by T cells. Proc Natl Acad Sci U S A. 1995; 92:11249-11253. (Biology).
  18. Kobata T, Morimoto C. CD27 Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:67-69.
  19. Kuijpers TW, Vossen MT, Gent M-R, et al. Frequencies of circulating cytolytic, CD45RA+CD27–, CD8+ T lymphocytes depend on infection with CMV. J Immunol. 2003; 170:4342-4348. (Biology).
  20. Malbran A, Belmonte L, Ruibal-Ares B, et al. Loss of circulating CD27+ memory B cells and CCR4+ T cells occurring in association with elevated EBV loads in XLP patients surviving primary EBV infection. Blood. 2004; 103:1625-1631. (Biology).
  21. Martorell J, Rojo I, Vilella R, Martinez-Caceres E, Vives J. CD27 induction on thymocytes. J Immunol. 1990; 145:1356-1363. (Biology).
  22. Maurer D, Fischer G, Fae I, et al. IgM and IgG but not cytokine secretion is restricted to the CD27+ Blymphocyte subset. J Immunol. 1992; 148:3700-3705. (Biology).
  23. Reiter C. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:341-342.
  24. Reiter C. T9. Cluster report: CD27. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:350.
  25. Sugita K, Dasgupta J, Mojima Y, Agematsu K, Schlossman S, Morimoto C. Protein kinase A-mediated phosphorylation of the T-cell surface antigen CD27. Immunology. 1993; 80:217-221. (Biology).
  26. Sugita K, Robertson MJ, Torimoto Y, Ritz J, Schlossman SF, Morimoto C. Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. J Immunol. 1992; 149:1199-1203. (Biology).
  27. Walker EB, Haley D, Miller W, et al. gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells.. Clin Cancer Res. 2004; 10(2):668-80. (Biology). View Reference
  28. Watts TH. TNF/TNFR family members in costimulation of T cell responses. Annu Rev Immunol. 2005; 23:23-68. (Biology). View Reference
  29. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
  30. de Jong R, Loenen WA, Brouwer M, et al. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol. 1991; 146:2488-2494. (Biology).
View All (30) View Less
340424 Rev. 1

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