Product Details
BD™
ITGAX; AlphaX integrin chain; Axb2; Integrin alpha-X; CR4; Leu M5; SLEB6
Human
Mouse BALB/c IgG1, κ
Spleen Cells from Human with Hairy Cell Leukemia
Flow cytometry
13 μg/mL
20 μL
IV N012
3687
RUO (GMP)
658330 Rev. 1
Antibody Details
B-ly6
The CD11c antibody, clone B-ly6, is derived from the hybridization of X63 mouse myeloma cells with spleen cells from BALB/c mice immunized with a cell suspension isolated from the spleen of a patient with hairy cell leukemia.
The CD11c antibody specifically binds to a 150-kilodalton (kDa) type I integral membrane glycoprotein. The CD11c antigen is also known as p150, integrin alpha X (integrin αX), and CR4.
658330 Rev. 1
Format Details
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
658330 Rev.1
Citations & References
Development References (17)
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Barclay AN, Brown MH, Law SKA, McKnight AJ, Tomlinson MG, van der Merwe PA. The Leucocyte Antigen FactsBook 2nd ed. San Diego: Academic Press; 1997.
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Frick C, Odermatt A, Zen K, et al. Interaction of ICAM-1 with β2-integrin CD11c/CD18: Characterization of a peptide ligand that mimics a putative binding site on domain D4 of ICAM-1. Eur J Immunol. 2005; 35:3610-3621. (Biology).
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Gower RM, Wu H, Foster GA, et al. CD11c/CD18 expression is upregulated on blood monocytes during hypertriglyceridemia and enhances adhesion to vascular cell adhesion molecule-1. Arterioscler Thromb Vasc Biol. 2011; 31:160-166. (Biology).
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Grouard G, Durand I, Filgueira L, Banchereau J, Liu YJ. Dendritic cells capable of stimulating T cells in germinal centres.. Nature. 1996; 384(6607):364-7. (Biology). View Reference
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Lishko VK, Yakubenko VP, Hertzberg KM, Grieninger G, Ugarova TP. The alternatively spliced αEC domain of human fibrinogen-420 is a novel ligand for leukocyte integrins αMβ2 and αXβ2. Blood. 2001; 98:2448-2455. (Biology).
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Myones BL, Dalzell JG, Hogg N, Ross GD. Neutrophil and monocyte cell surface p150,95 has iC3b-receptor (CR4) activity resembling CR3.. J Clin Invest. 1988; 82(2):640-51. (Biology). View Reference
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O'Doherty U, Peng M, Gezelter S, et al. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.. Immunology. 1994; 82(3):487-93. (Biology). View Reference
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Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
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Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:517-542.
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Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). View Reference
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Stacker SA, Springer TA. Leukocyte integrin P150,95 (CD11c/CD18) functions as an adhesion molecule binding to a counter-receptor on stimulated endothelium. J Immunol. 1991; 146(2):648-655. (Biology). View Reference
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Thomas R, Lipsky PE. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells.. J Immunol. 1994; 153(9):4016-28. (Biology). View Reference
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Visser L, Shaw A, Slupsky J, Vos H, Poppema S. Monoclonal antibodies reactive with hairy cell leukemia. Blood. 1989; 74(1):320-325. (Biology). View Reference
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Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
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van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
658330 Rev. 1
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