CD8 PerCP

BD™ CD8 PerCP

Name: CD8 PerCP
Brand: CD8 PerCP
SKU: 345774

Clone SK1 (also known as Leu-2a; Leu2a) (CE_IVD)

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Scientific Resources

Catalog No:345774

Unit:EA (1 Each)

Size:

100 Tests

Product Details

Brand: BD™

Alternative Name: CD8α; CD8A; CD8 alpha; Leu2a; MAL; T8; p32

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Peripheral Blood T Cells

Application: Flow cytometry

Concentration: 6.25 µg/mL

Vol. Per Test: 20 μL

Workshop Number: I T51,74; III T118,152,571

Entrez Gene ID: 925

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: CE_IVD

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

Data Sheets

Safety Data Sheet

Instruction For Use (IFU)

345774 Rev. 1

Antibody Details

SK1

CD8 is intended for in vitro diagnostic use in the identiﬁcation of cells expressing CD8 antigen, using a BD FACS™ brand ﬂow cytometer. The ﬂow cytometer must be equipped to detect light scatter and the appropriate ﬂuorescence, and be equipped with appropriate analysis software for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

345774 Rev. 1

Format Details

PerCP

PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP

Excitation Max: 481 nm

Emission Max: 675 nm

345774 Rev.1

Citations & References

View Development References

Development References (2)

Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference

345774 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.