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BD Multitest™ 6-Color TBNK Kit

BD Multitest™ 6-Color TBNK reagent with optional BD Trucount™ Tubes is a six-color direct immunofluorescence reagent for use in identifying and determining the percentages and absolute counts of T, B, and natural killer (NK) cells, as well as the CD4+ and CD8+ subpopulations of T cells, in peripheral blood on a BD flow cytometer.


Determining percentages or absolute counts of CD3+CD4+ T lymphocytes is used in monitoring human immunodeficiency virus (HIV)-infected individuals. Individuals with HIV typically exhibit a steady decrease of CD3+CD4+ T-lymphocyte absolute counts as the infection progresses.1


Determining percentages or absolute counts of CD3+, CD3+CD4+, or CD3+CD8+ T lymphocytes or CD19+ B lymphocytes is used to characterize or monitor some forms of immune deficiency and autoimmune diseases.1,2


Determining percentages or absolute counts of CD3– and CD16+ and/or CD56+ NK lymphocytes is used in immunological assessment of hematologically-normal subjects or patients having, or suspected of having, immune deficiency or other immune-mediated diseases.1,3


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Why BD Multitest™ 6-Color

  • Performs lymphocyte enumeration of mature T, B and NK cells in a single tube
  • Provides the option of absolute counts when paired with BD Trucount™ Tubes
  • Works in a fully integrated system with BD FACSDuet™ Sample Preparation System for automated sample preparation allwoing a complete automated workflow from sample to answer, reducing hands-on time and error-prone steps.
  • Can be used with BD FACS™ Universal Loader for automated sample acquisition on BD FACSLyric™
  • CE-IVD assay modules with built-in templates and automated gating to work on BD FACSLyric™ instrument software for setup, QC, acquisition analysis and reporting. Seamless integration of data reporting of test and patient results through bidirectional LIS connectivity.

The BD Multitest™ 6-Color TBNK Kit Simplifies Your Workflow and Increases Productivity

  • An easy-to-use Solution
    With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. The reagent includes a cocktail of multiple fluorescently labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining.

  • Accurate cell counts with BD Trucount™ Tubes
    BD Trucount™ Tubes help to ensure more accurate cell counts - requires no additional pipetting steps, precise and reproducible. The tubes contain a lyophilized pellet of fluorescent beads that serves as an internal standard. BD FACSuite™  Clinical Application automatically calculates lymphocyte absolute counts and subset percentages.

  • Improve safety, reduce hands-on time
    The BD Multitest™ 6-Color TBNK reagent employs a no-wash formula that reduces tube handling. Processing fewer tubes in less time may decrease potential exposure to biohazardous material, improving safety for laboratory personnel.

  • Increase productivity

    Together with the BD FACSLyric™ cell analyzer and BD FACSuite™ software, the BD FACS™ Universal Loader option and the BD FACSDuet™ Sample Preparation System, the BD Multitest™ 6-Color TBNK reagent provides a fully automated, standardized and efficient workflow for lymphocyte enumeration to best suit your routine immunophenotyping needs.



The BD Multitest™ 6-Color TBNK reagent with Trucount™ tube for accurate and reliable enumeration of mature T, B and NK cells.

 BD FACSDuet™ Sample Preparation System, BD FACS™ Universal Loader, BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ applications and  BD FACSuite™ Clinical BD Multitest™ 6-Color Assays are in vitro diagnostic medical devices bearing a CE mark.


 BD Multitest™ 6-Color TBNK and the optional BD Trucount™ Tubes are in vitro diagnostic medical devices bearing a CE mark and are CE certified by BSI Group The Netherlands B.V. (Notified Body Number = 2797).


BD Flow Cytometers and BD FACSDuet™ Sample Preparation System are Class 1 Laser Products. 



  1. Cossarizza A, De Biasi S, Gibellini L, et al. Cytometry, immunology, and HIV infection: three decades of strong interactions. Cytometry. Part A: the Journal of the International Society for Analytical Cytology. 2013;83(8):680-691.
  2. Hanson IC, Shearer WT. Ruling out HIV infection when testing for severe combined immunodeficiency and other T-cell deficiencies. J Allergy Clin Immunol. 2012;129(3):875-876.e875
  3. Oliveira JB, Fleischer TA. Molecular- and flow cytometry-based diagnosis of primary immunodeficiency disorders. Current Allergy and Asthma Reports. 2010:10(6):460-467.