The application of electrostatic cell-Sorting in Pediatric Oncology Translational Research
In pediatric oncology translational research, every cell counts. Researchers face the unique challenge of working with extremely limited biological material compared to adult-derived specimens. To bridge the gap, high-efficiency cell sorting is not just a luxury—it is a necessity.
Join Dr. Alessandra Cianflone as she provides a comprehensive overview of electrostatic cell-sorting methodologies. Using the BD FACS Melody™ cell sorter as a reference, Dr. Cianflone will share strategies to ensure reproducible sorting of rare populations from pediatric samples, where biological material is extremly limited.
What You Will Learn:
Efficiency with Scarcity: How to manage specimen type and condition to maximize cell viability, cellularity, and final yield from limited pediatric samples.
Tackling Solid Tumors: Explore optimized methods for isolating aberrant cell populations from complex tissues, such as pediatric brain tumors.
Sample enrichment for scRNAseq: Why sorted cells provide higher sequencing efficiency, lower background, and better detection of rare populations.
Speaker
Dr. Alessandra Cianflone
Senior Researcher, UOC Clinical Translational Research
AORN Santobono-Pausilipon
Based in Naples, Italy, Dr. Cianflone brings years of multidisciplinary expertise in pediatric oncology and biobanking. Her work focuses on translating laboratory findings into clinical realities for the most vulnerable patient populations.
