BD FACSymphony™

Customized solutions for high parameter cell analysis

Customized solutions for high parameter cell analysis

The BD FACSymphonyTM system is a novel cell analyzer that leverages the inherent benefits of flow cytometry and enables the simultaneous measurement of up to 50 different characteristics of a single cell. This high parameter flow cytometer is a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations.

Performance Customization Specialized Support

Customizable models provide flexibility for your research

  • Configure the BD FACSymphony TM system to your needs today with room for growth tomorrow
  • Up to 50 detection parameters (including FSC and SSC) featuring decagon arrays for up to 10 parameters on a single laser line are available
  • Select and configure up to a maximum of 10 lasers* from various wavelengths with multiple power ratings

Driving deeper scientific insights

This advanced instrument features an ultra-quiet VPX electronics system that supports up to 50 high-performance photomultiplier tubes (PMTs) and improves detection sensitivity to enable you to identify and analyze rare cell types and events. The capabilities of this platform technology uniquely allow you to conduct deep and broad phenotyping and gain richer scientific insights by fully leveraging the broad portfolio of BD Horizon Brilliant™ reagents.

When analyzing panels designed with BD Horizon Brilliant dyes, this platform helps you to overcome research challenges, such as collecting maximal information from a precious sample, and increases lab throughput with broad phenotyping panels that combine multiple cell-line-specific panels.

This highly customizable platform can be configured so that you can select from multiple laser wavelengths and power ratings, and choose the positions of decagon detection arrays to address the requirements of your specific research application.

Custom optics for your application

Innovation in detection-array technology has allowed for a decagon formation to detect 10 parameters on a single laser line. The arrays can be configured on the laser of your choice.

FACSSymphony Features - Customization Laser Choices

As a Special Order Research Product (SORP), the BD FACSymphony™ system offers 25 laser wavelengths to optimally configure your instrument for your specific research application. Additionally, there are multiple power ratings for most lasers that can be adjusted, stored and recalled using the digital laser command and control functionality.

Fluorochrome availability and excitation characteristics across various wavelengths should be discussed during the configuration process to identify the best use of reagents for your research. Optimal laser power settings for certain fluorochromes may be available.

All BD FACSymphony TM systems come with access to reagents and specialized support to get you up and running as quickly as possible. This includes onsite and offsite support from our team of dedicated high parameter application specialists to discuss your research goals and consult with you about reagent choice to simplify your panel-design activities.


The reagents provided with the purchase of a BD FACSymphony instrument will assist you in setting up your instrument, identifying spectral characteristics when running various fluorochromes simultaneously and beginning the design of your initial panels. The reagents will include a fluorochrome evaluation kit, which is a suite of human CD4 SK3 reagents in nearly every color option to evaluate the detection capabilities of your custom BD FACSymphony system configuration. The kit also includes samples of specificities on the color of your choice for your research needs. Where available, reagent access includes high concentration, mass-size human reagents to avoid dilution effects in high parameter cocktails.

As part of the program, you will be given a dedicated point of contact for ordering reagents, so that you can contact a specialized applications team who can answer your high parameter cell analysis questions.Contact  BDB_HPS_VIP@bd.com for all your high parameter application needs.

Broad portfolio of high-quality dyes and conjugates expand experimental design options

BD’s broad portfolio of fluorochromes featuring the BD Horizon Brilliant™ dyes offers flexibility for experimental design. Leverage the principles of antigen density and relative fluorochrome brightness to optimally design your panel.


BD OptiBuild™ custom reagents offer on-demand access to hundreds of specificities associated with a range of BD Horizon Brilliant dyes and are available in small sizes with quick turnaround times. This new portfolio of conjugates complements the existing catalog reagents that previously had limited color options with a wide selection of cell-surface antibodies. Please contact BDB_HPS_VIP@bd.com to explore how you can simplify the addition of markers to your experiments for your high parameter custom solutions.

FACSSymphony Reagents - Table

Prototypes of BD Horizon Brilliant™ dyes (-P)

BD Life Sciences is committed to continuing to develop new BD Horizon Brilliant dyes across various laser lines to improve the spectral properties of dyes and minimize the need for compensation in higher-order panels.

BD FACSymphony™ system owners receive early access to a suite of prototype dyes for use in high parameter panel design. Although these dyes are near completion and have received initial quality specifications, they may undergo additional development that could result in minor performance changes. The –P nomenclature indicates the prototypic nature of the dyes, and any significant changes to the structure of the dye to optimize performance will be appropriately communicated to customers.

Contact BDB_HPS_VIP@bd.com for all your high parameter application needs.

FACSSymphony Reagents - Spectra

Class 1 Laser Product.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

*Dependent on laser choice

Note: For specificities not yet available in the catalog or through BD OptiBuild™ custom reagents, high parameter users have access to a small-scale custom conjugation program for BD Horizon Brilliant dyes, including early access to the high parameter dye menu described above.

Alexa Fluor® is a registered trademark of Life Technologies Corporation.

Cy™ is a trademark of GE Healthcare.

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